Lutz, Triploid Mutants in Oenotherti. ',\ ( .\~ t 



5484 X 5483 (two individuals of No. 4, culture 6048, crossed), 

 "produced 7 offspring, no two of which were alike. These have 

 been described as follows: 1. Stiff, ovate, uncrinkled, grayish leaves, 

 spreading in centre of rosette. 2. Rather firm, crinkled leaves, 

 broadly oblong, slightly apiculate, erect in centre of rosette. 3. Resem- 

 bles 0. nanella, but with oval, rounded leaves. 4. Leaves large, 

 but with very broad crinkled blades and winged petioles. Grayish 

 pubescent. 5. Resembles linear-leafed yiyas. Leaves nearly horizontal, 

 light green, slightly grayish, uncrinkled. 6. Similiar to 1, but leaves 

 less acute, less grayish, and a little more ascending. 7. Broadly 

 oblong, acutish, crinkled, grayish. Centre of rosette moderately 

 erect, but large leaves nearly horizontal. 



5558 X 5483 (No. 5, culture 6051 X one individual of No. 4, 

 culture 6048 -- same male used in the cross just described), "produced 

 7 offspring, no two of which were alike, but most show some resem- 

 blance to the several forms otO.gigas. These have been described 

 as follows: 1. Thick, dark green, ovate leaves, almost uncrinkled. 



2. Very crinkled, looks intermediate between gigas and lata. 



3. slightly resembling 0. Lamarckiana, but with darker green leaves 

 of thicker, fleshier texture. 4. Could easily be classed as a moder- 

 ately broad-leafed gigas. 7. Rather young, but appears now similar 

 to 0. Lamarckiana of same age." 



Nos. 5 and 6 were not characterized. They were probably 

 too young. 



The second mutant, 5589 (having a chromosome number in 

 the region of 21), self-pollinated, produced but one offspring of the 

 5509 type mentioned among the progeny of 5445. 



The offspring of 5589 X 0. yiyas need not be considered here. 



As was previously stated, no germinations were obtained from 

 the seeds of the 22-chromosome mutant 4453. 



From the fore-going it is clear that the 21-chromosome mutant 

 does not breed true with respect to vegetative character. Unfor- 

 tunately, nothing is known of the chromosome numbers of these 

 plants, as the opportunity for this study was not afforded. While 

 too much weight must not be attached to speculations in regard 

 to the chromosome numbers of these offspring, some statement as 

 to the probabilities may not be amiss. 



So far as I have observed (as previously stated), I have found 

 that all individuals of a given type of vegetative character invariably 

 have identical somatic chromosome numbers, regardless of the diversity 

 of origin of the individuals in question. If the 75 offspring of 

 5483 self-pollinated which were 'apparently good 0. Lamarckiana' 

 in the early rosette stage maintained these characters throughout 

 their life histories, and were in every way indistinguishable from 

 0. Lamarckiana, I think it is probable that each had 14 chromosomes. 



