62 



ROPINESS IN WATTLE BARK IXPUSIONS 



Table v. — Sugars, etc. with 0-5% Tannic Acid. 



Phase 



A2 



B2 



Source of Nitrogen Asparagin 



Ammonium 

 Sulphate 



Asparagin 



Ammonium 

 Sulphate 



Days at 28° 



6 i 10 



6 10 



6 10 



6 10 



1. Dextrose 



2. Levulose 



3. Saccharose 



4. Maltose 

 o. Galactose 



6. Raifinose 



7. Mannit 



8. Control 



(0 



O O 





 O 



s 

 o 



o 







s 

 o 



o 



( o 



o i o 



i. ( o 



^ is 



o 



+ 







s 

 o 







4- 

 o 







o 



s 

 o 



o 



o o o 

 o o s 



10 o 



On the 4tLi tlav, the ammonium sulphate tests of A2 were phited to see how 

 far the pliases liad altered. 



Table va. — Percentage of Phase A2 in Ammonium Sulphate at 28° 



In all oases, except mannit, the cohesive pha.se A2 had become, by the fourtli 

 day, more or less altered to the ditt'use phase Al, and it is rather c-vtraordinary 

 that those with a maximum proportion of the diffuse ])liase should have been ropy. 

 It is possible tiiat the ropy substance was formed before the alteration to the dif- 

 fuse phase occurred and the gradual suppression of the phase A2 as shown by 

 the relative numbers on the 10th day, bears out this idea. Another jieculiarity 

 is that while the maltose test with asparagin gave no ropiness, and with ammonium 

 sulphate a jjronounced ropiness <m the 4th day, smears made on that day showed 

 the same proportion of A2, viz. 35 %. From these proportions it would appear 

 tliat ropiness has less to do with tho phase of the organism than the previous tests 

 had led one to believe, but as on the 10th day, the asparagin tost contained 1 % 

 of A2, and the ammonium sulphate test 25 %. there is still the suspicion that 

 some relation exists. 



