BY E. GREIG-SMITH. 83 



A2 and A2a showed a filiform canal with an npper portion waved and hearing' a 

 flat nail-head. In five davs the nail-head had become a napiform softened area. 



Nutrient tjelatin eoloires. — Al gave colonies showing an irregular, granular, 

 ivy-leaf-like structure in a shallow depression of softened gelatin. By the fifth 

 day the gelatio had H(|ueflcd and the growth had broken up into irregular scat- 

 tered grannies. A2 and A2a liquefied the medium slowly, and the colonies re- 

 jnained as moruloid or frog-spawn-like masses of irregular gi'anules. 



Glucose gelatin colonies. — The phases were all much the same, and this ap- 

 plies to all media with sugar. Al gave pale yellow colonies with i-aised centres 

 and raised circular margins (button-shape) ; they were about 7 mm. diameter in 

 four days. A2 grew as irregular moruloid masses, 3-5 mm. in diameter. Both 

 phases softened the gelatine. 



Dextrose agar. — Al gTew as a smooth raised colony of ropy consistency; A2, 

 dome-shaped, with or without a rugose margin, and the consistency wa.s rubber- 

 like rather than ropy. 



Bouillon. — A pronounced surface film and slightly turbid medium with a 

 faint deposit excepting when a film has fallen down. A2 causes the upper layers 

 of medium to be ropy. Nitrates are reduced to nitrites, indol is formed and 

 anmionia is produced. 



Potato. — A scanty, glistening, pale buff growth. 



Starch. — Faint saccharification occurred. 



Litmus-milk. — The medium was unaltered. 



Litmus broth icith sugars, etc. — Saccharose and dextrose gave acid and gas. 

 Mannit showed a bleaching only, lactose was unaltered. 



Classification number. — 221 .131.3523. 



Bacterium B2. 



Morphologg. — As A2, but a little stouter rod, 0.(1^. 



Nutrient Agar Stroke. — As A2. 



Nutrient Agar Colonies. — A corrugated, dome-shaped colony smaller than A2, 

 in appearance like a minute white raspberry. Microscopically, the colonies on 

 thickly sown ]ilates show a granular central area bounded by an irregular, dark, 

 ivy-leaf shaped band outside which and half way to the edge there is a dark 

 circular ring; otherwise the colony structure is coarsely granular. The freely- 

 growing colonies have often rosette or spoke-like marking's extending from the 

 centre to the repand edge, but the typical structure is mesenteric. 



Nutrient gelatin stab. — As A2, but the liquefaction is very slow. 



Nutrient gelatin colonies. — As A2. 



Glucose gelatin colonies. — As A2, but Ihey do not liquefy the medium. 



Dextrose Agar. — As A2. 



Bouillon. — As Al, but the film is flakey. 



Potato. — A glistening white growth. 



Starch. — As A. 



Litmus milk. — As A. 



Litmus broth with sugars, etc. — As A. 



Classification, number. — As A. 



The two bacteria have some resemblance to Bac. Atherstonei, the variable 

 galactan bacterium described by me as having been obtained from the tissues of 

 Strgchnos Atherstonei.* That organism exhibited two phases. The colonies in 

 glucose-gelatin grew as brittle transparent masses, apparently containing a brittle 



•These Proceedings, 1904, 442. 



