45-J 



CYANOGENESIS IX PLANTS, 



in a ciUTent of st'am and the distillate received in a solution of sodium hydrox- 

 ide. After distilling for one hour, the contents of the flask were acidified to 5 % 

 with sulphuric acid, and the distillation continued. In some cases the enzj-me was 

 destroyed by treating the leaves directly with boiling 5 ':''c sulphuric acid and 

 immediate distillation. 



Besitlts. — The hydrocyanic acid obtained is expressed as nulli'.;ranis in 100 

 gTams of iilant-material dried at 100° C. 



Tablk a. 



/h'lii'/ tHs/i//afioii of /eai'e.s. 



Exp. Time. 



Particular treatment. 



HCX. 



Distilled with water alone in steam cur. 

 ,, with .5 Or sulphuric iicid 



Total in H hrs., unfiuished- 



10 



hr. 



Distilled with water alone in steam 

 with 5 % sulphuric acid 



Total in 41 hrs., iinfinished- 



none 

 none 

 0-'5 



o-r> 



30 



•to 



hr. 



Distilled with water alone without steam 

 with ."> % sulphuric acirl 



none 

 2 



2 

 2 

 2 



none 



Total in 5 hrs., finished- 



8 



Added boiliny: '> Or sulphuric, distilled 

 in current of .-iteam 



Total in 4 hrs., finished- 



13 



Sample II. 



cc.ntaining 



328 m!,'s. f^ 



HCN. 



6 ; 1 hr. 

 1 .. 

 1 ,, 



1 hi 



Distilled with water alone in air-current 



.\dded boilinj; li '/r sulphuric, distilled 

 in air-current 



Total in 3 Ins.. unfinished 



