50 CULTUEAL STUDIES OF SPECIES OF PENICILLIUM. 



The same organism has been found once in accidental culture in this 

 laboratory; received once from a correspondent in Halle, Germany, 

 and later found under this name as No. 1179 in De Thumen's Myco- 

 theca Universalis; collected by Ravenel in South Carolina in 1876 

 upon leaves of Buxus; this and several other specimens were found 

 in the mycological collection of the Bureau of Plant Industry, United 

 States Department of Agriculture. The spores are the same length 

 as given by Saccardo, but slightly broader. The number of specimens 

 found under this name from widely different workers appears to 

 justify the belief that this is the organism described by Link under 

 this name. If the development of a mucilaginous mass enveloping 

 the conidia be regarded as a sufficient basis for separation of such 

 species under the generic name of Gliocladium, this species would 

 become Gliocladium roseum. (Link). 



The form upon Buxus is cited by Saccardo, referring to it as U P. 

 roseum Cooke, non-Link," and held to be Verticillium buxi Auersw. 

 et Fleisch. Examination of the material would indicate that in 

 De Thumen's collection at least this species is more closely allied to 

 the other species of Penicillium than to Verticillium. 



CULTURAL DATA. 



Color white or shades of salmon pink; reverse cream or white; color in media, 

 none. 



Odor, none. 



Fifteen per cent gelatin in water, medium growth, white. Liquefaction, rapid. 

 Litmus reaction, alkaline, strongly. Potato agar and bean agar, good growth, but 

 white. Potato plugs, white colonies. Cohn's solution, slight growth. 



In Dox's solution, with butterfat as a source of carbon, this species caused drops 

 of yellow oil to separate out. 



At 37° C, killed; check at 20° C, good. 



PENICILLIUM CAMEMBERTI Thorn. 



Emended from U. S. Department of Agriculture, Bureau of Animal Industry, Bui. 

 82, p. 33, fig. 1, 1906. 



Possible syn.: P. album Epstein (not Preuss), Archiv f. Hyg., Bd. 45, Hit. 4, 

 p. 360, 1902. 

 P. epsteini Lindau, Deutschl. Krypt. Flora, Pilze, VIII, p. 166. 



Colonies on potato agar or lactose gelatin effused ; white (sometimes yellowish white), 

 changing in 5-8 days to gray -green (glaucous); surface of colony floccose, of loosely 

 felted hyphse about 5/< in diameter, reverse of colony yellowish white; conidiophores 

 300-800/i in length, 3-4/i in diameter, septate, cells thin-walled, often collapsing in 

 age, arising as branches of aerial hyphae; fructification sometimes 175/i in length, but 

 usually much less, consisting commonly of one main branch and one lateral branch, 

 sparingly branched to produce rather few conidiiferous cells which bear long loosely 

 divergent chains of conidia. Conidiiferous cells 8-11 by 2.4-3/z. Conidia at first 

 cylindrical, then elliptical, and finally globose when ripe, smooth bluish green by 

 transmitted light, thin-walled and commonly guttulate, 4.5-5.5/* in diameter, swelling 

 in germination to 8-10/t. Germ tubes one to several. Cells of mycelium about 5 

 by 20-40/*. Liquefies lactose gelatin only under center of colony. Produces a strong 



