PENICILLIUM NO. 37. 63 



The whole fructification appears in this way double, t riplo, or quadruple or even 

 more complex by a secondary verticil from the central branch. Conidiiferous cells 

 6-7 by 2-3/1. Conidia globose when ripe, 2 I 3^ (even 3.5/a diam. in cane-sugar cul- 

 tures) in diameter, bluish-green, Blightly granular in contents, adhering in chains 

 in fluid mounts, losing vitality rapidly with change of color in old colonics. Colonies 

 liquefy gelatin rapidly, so thai they lie in pool.- of liquid within a week. Litmus 

 reaction in plain gelatin, Btrongly alkaline. Produces a lemon-yellow color soluble 

 in alcohol in media containing Bugars, milk, gelatin, bread, and potato. 



Found in cultures from milk and cheese, probably cosmopolitan. 



Could this be P. dtreo-niffrum Dierckx? 



CULTUKAL DATA. 



Color bluish green, becoming dark brown when old if exposed to light; reverse 

 colorless or yellowish; color in media lemon-yellow when cane sugar or gelatin or 

 peptone is present, none in some media. 



< >dor, none. 



Fifteen per cent gelatin in water, characteristic growth, becoming white by sec- 

 ondary sterile mycelium; liquefaction rapid, colonies floating in 5-(» days; litmus 

 reaction alkaline. Potato agar and bean agar, colonies spreading more than upon 

 gelatin, agar not colored or slightly colored. Potato plugs, typical growth, potato 

 colored yellow, with yellow drops transpired; Raulin's fluid, typical growth, very 

 slight yellow color. < John's solution, good growth, no yellow color. 



Synthetic fluid (Dox's), carbon supplied as: Cane sugar, good growth with yellow 

 color up to 30 per cent, with acid reaction. Lactose 3 per cent, slow poor growth 

 giving a violet tinge instead of yellow color. Lactic acid 0.9 per cent, slight growth. 

 Levulose 3 per cent, small characteristic growth. Galactose 3 per cent, good growth, 

 reaction neutral or slightly acid. Glycerin 3 per cent, very small colonies, no color 

 in medium. Alcohol, 5 per cent, good growth, no yellow. Potato starch 3 per cent, 

 good growth, slight if any yellow. Tartaric acid, slow colorless colony. Butterfat, 

 rich growth, lemon-yellow fluid. 



Milk, rapid growth; curdling (0.25 per cent calcium chlorid added) in 10 days; 

 digestion rapid; coloration pale yellowish. 



At 37° C, slow growth, white colony, no color in medium; check at 20° C, rich 

 growth, green, yellow in medium, in bean agar with cane sugar. 



PENICILLIUM No. 37. 



(Var. of P. citrinumt Or allied to P. citrinumt) 



Colonies in media without sugar, green, gray-green, or gray; with sugar persistently 

 green ; surface velvety strict, composed of short crowded conidiophores up to 100/x in 

 length, branching from closely woven mycelium partly submerged, partly aerial, 

 with margin narrow, nol widely spreading in the substratum; reverse of colony and 

 substratum nol colored or creamy ; coni dial fructifications sometimes a single verticil 

 of conidiiferous cells, sometimes 2 to 1 verticillate branches; chains of conidia from 



each verticil forming a <olunm up to ;>(ii> i;niw ( in length in sugar dia; branches 



of fructification 13 II by 2 2.5^ enlarged a1 apex; conidiiferous cells 8 10 by 2+^ 

 abruptly narrowed into sterigmata, usually ti It) in each verticil; conidia broadly 

 elliptical to globose, 2.5-3/* al first becoming I '><< before germinating, thin-walled, 

 smooth, pale yellowish green, germinating by a single tube; colonies liquefy gelatin 

 rapidly (6 to 7 days), with strong alkaline reaction to litmus. 



Received from Prof. P. II. Rolfs, .Miami, I'la., in culture upon bean stems, 1905. 



Allied to P. cilrinum by morphology and culture reactions, but differing in lacking 

 the power to color media yellow ami in its greater dependence for typical growth 

 upon the presence of cane sugar. 



