I'l.Xh'lU.ir.M A.TRAMENTOSUM. 65 



Bpondence in reactions, withoul identity; this form appears to be much more 

 dependent upon cane sugar for the production of typical color of the conidia and 

 growth than is P There is al eater tendency to the production 



of a layer of mycelial hyphse jusl above the surface of the substratum, from which the 

 conidioph ires arise as aerial branchi 



This form was received from Prof. C. E. Marshall, Agricultural College, Michigan 

 under i be uame of /'. glaucum. 



(Mil i: Al DATA. 



Color pale blue-green; reverse of colony cream, aol colored; color in media, none. 



( (dor, uone. 



Fifteen per cenl gelatin in water, rather small pale blue colonies, rapidly becoming 

 white by secondary sterile growth of hyphse; liquefaction, rapid- 6 days; Litmus 



reaction, alkaline. Potato agar, as in gelatin. Potato plugs, very p ■ growth, 



grayish or yellowish green. Raulin's Quid, slow bul typical colonies, delicate l>lue; 

 Cohn's solution, slow and restricted grow th. 



Synthetic fluid (Dox's), carbon supplied as: Cane Bugar, grows well in concentra- 

 tions up i" 60 per cent. Lactose 3 per cent, small colonies Lacking nourishment. 

 Lactic acid 0.9 per cent, small colonies floating in fluid. Le> ulose 3 per cent, good 

 growth, alkaline reaction. Galactose 3 per cent, good growth, alkaline reaction. 

 Glycerin 3 per cent, slow-growing colonies, becoming gray-brown when old. Potato 

 starch, good colonies. Butterfat, b1o\s and ill nourished growth. 



Milk, rapid growth; curdling (0.25 per cent calcium chlorid" added) in 7 days; diges- 

 tion rapid and very complete; color in milk, none. 



At 37 C . killed in 6 days; at 20° C, good growth. 



PENICILLIUM ATRAMENTOSUM n. sp. 



Latin diagnosis. — Coloniis in gelatina vel agaro Solani tuberosi au1 phaseoli cultis, 

 viridibus, parte aeria plerumque ex conidiophoris singulatim orientibus, medio cum 

 hyphis aereis interspersis, margine alboexhyphis fertilibus angusta; reverso incolorato 

 vel parum ochraceo; substrato au< incolorato au1 in substratis saccharin is et in lacte 

 atrobrunneo tarde fere atro; conidiophoris 240 300 usque 400/i Longis; fructibus conidi- 

 cie LOO usque 200/i longis, ram is I 2 \ erticillatis 2-4 insequaliter Longis in verticillo in 

 apice incrassatis; basidiis v L0/i Longis, parallelis in verticillo; cutcnis conidiorum 

 eodem verticillo in columno compactis; conidiis ellipticis, 3.5 I (usque L.8) X2.5-3 

 usque 3.5/«, laevibus, viridibus, 6— 7/t incrassatis e1 uno tubo germinantibus; coloniis 

 gelatinam cito Liquefacientibus, alkalinis lacmo; odore in lacte proprio, in substratis 

 aliis aullo. 



Ex caseo cull tun. Siori ' onn., 1905. 



AHine P. citrino. 



Colonic- upon gelatin or upon potato or bean agar bright green, aerial pan mostly 

 of simple conidiophores, mixed in older parts with branching aerial hyphse bul nar- 

 rowly spreading al the margin by new conidiophores onlj . Reverse of colonies shows 

 a slight production of yellow (ochraceous) color. Conidiophores 240 100/t, averaging 

 about 300// in length. Conidial fructification up to 200/i in length, usuallj LOO/t or 

 le.-.-. verticillatelj or twice verticillately branched; branches 2 I in a verticil di- 

 vergent, unequal in length, swollen al ends, bearing conidiiferous cells. Theconjdial 

 i bains from each verticil form a dene column, which diverges more or Less from the 

 other columns when old. Conidiiferous cells 8 LO/t in length, closely parallel. Conidia 

 elliptical, varying from 3.5 [ft by 2.5 3/i on omewhal Larger in gelatin cultures, 



up to L8 bj 3 5/i, smooth, homogeneous green with a slight yellowish made when seen 

 in mass, swelling to 6 7<< in diameter and germinating bj a single tube. Mycelial 

 cell- 5 7/< in diameter and up to 30// or more in Lengt h. « oloniee Liquefy BUgar-gelatin 



8108— Bull. 118—10 5 



