SPECIES FOBMING PINE SCLEROTIA. 



79 



media with cane sugar produce Bclerotia more aumerously and more quickly (5 da 

 than without sugar. Butterfal as a sourceof carbonin Dox'sfluid, typical colonies. 

 Milk, typical; curdling (0.25 per cenl calcium chlorid added I in 9 days; digestion, 

 Blow; color in milk, none. 



Pen n m X". 30. 



Colonics on sugar gelatin and potato or bean agar gray-green or green persistently, 

 Burface growth mostly of crowded conidiophores and producing pink Bclerotia al the 

 surface or parti) embedded in thesubstratum L50 300u in diameter, broadly spreading. 

 Conidiophores from 240 525>, usually aboul 300/i, in length either arising separately 

 from the substratum or as branches verj close to its surface. < lonidiaJ fructification 

 a single verticil of conidiiferoue cells bearing conidia in a close column up to500/i in 

 length by L"> 30/z. Conidia elliptical or Bubglobose 2.5 by '■[■■ oiZfi, withaslighl green- 

 ish color. Colonics liquefy sugar gelatin rather -lowly, and give an acid reaction 

 with litmus. 



Fig. M.—Penicilliuin No. 29: a, b, conidlophore and verticil of conidiiferous cells (X 'J00); c,d, e, germina- 

 tion of conidia i < 900);/, g, h,j, sketches of conidia! fructifications i < L40); /.. diagrammatic sketch 

 from photomicrograph showing relations of sclerotia and conidial fructifications. 



Apparently related to No. 29, bu1 differing in the length and density of its column 

 of conidia, in the position of the sclerotia. in habit, in culture, and in its acid reaction. 

 Collected at Storrs, Conn., upon decaying Laeiariua vellereus, September, li>04. 



CULTURAL HA l \ 



Color green or grayish green, persistently, with abundanl pink sclerotia; reverse 



UUColored; color in media, none. 



( >dor, none. 



Fifteen percent gelatin in water, typical; Liquefaction rather -low; litmus reac- 

 tion acid. Potato agar and bean agar, typical. Potato plugs, typical. Cohn's 

 solution, germination only. 



Synthetic fluid (Lox'si, carbon supplied as: Cane sugar, rich growth. Lactose 3 

 per cent, slowly typical colonies, acid reaction. Lactic acid 0.9 per cent, weak 

 growth. Levulose 3 per cent, medium growth. Galactose 3 per cent, typical. Gly- 

 cerin :; per cent, very weak growth. Butterfat, slow , bul characterisl ic colonies. 



Milk, curdling (0.25 per cent calcium chlorid added I in 9 days; digestion, slow but 

 complete; color in milk, none. 



At 37° C, no growth; grew when cooled; check al 20° C, typical. 



