(3 Stop es and Fujii, The nutritive relationa of the Burrounding tissues etc. 

 qaantities in the endosperm. Thal the cells of the endosperm were 



rieh in BUgai was eslablished by heating drops of sap obtained 

 ft tun thcin by tueans of capillary luhes, with an aqueous Solution 

 of acetate of Phenylhydrazin after the niethod of one of us 1 ). 01 

 l>\ heating sections of the tissues with the same reagenl in small 

 (ipcn tnbes. On cooling, the characteristic crystals of gluc-ozozone 

 developed in Large quantities, proving t hat there mnst bave been 

 iniicli glucose in the tissues. Cane sugar did not seem to be 

 present to any recognisable extent. To test this, sections were 

 placed in a strong aqueous Solution of Invertin for l 1 /^ hours aud 

 then tested with acetate of Phenylhydrazin, luit there was no 

 noticeable increase in the qnantity of ozozone crystals formed; 

 while iu the control experiraents carried on at the same time with 

 a weak Solution of cane sugar with the addition of iuvertin even 

 after 10 minutes we gol very well marked foimation of glucozozone 

 crystals. 



Oxydases were present in the vascular bnndles of the Integu- 

 ments, apparently in the pliloem, perhaps coinciding with the 

 "Leptomin" had been found by Kaciborski 2 ) in this position 

 in uiany plants. In the jacket cells it appeared also to be 

 pesent, but was difficult to demonstrate exactly owing to the 

 presence of the already rneutioned reducing substance in the 

 surrounding endosperm cells which we found hindered the reaction 

 of guajac resin. 



In other materials of an allied species of Ceratozamia just a 

 little older than the above, the same facts lield good, except that 

 the carbohydrates were not only present in a soluble form (sugars) 

 but also had begun to be deposited in the form of small starch 

 grains in the endosperm cells towards the base of the Archegonia. 



Protein substance also appeared to be supplied in a soluble 

 form at this stage, the nucellus showed very strong protein reactions 

 with Mi Hon 's reagent, Iodine (which was applied in alcoholic Solution 

 to sections previously treated with absolute alcohol to prevent the 

 reaction of starch) and biuret reaction. In the general endosperm 

 cells the reaction was faint though definite. while in the jacket 

 cells and their nuclei the reactions were niore apparent. In this 

 stage we could not observe any definite granules such as were 

 readily detectable in the later stages when protein storage begins 

 in the endosperm. In many Cycads the deposition of protein 

 substance in solid form appears to lag behind that of the starch 

 in and near the egg, but this seems to be reversed in the case 

 of Ginkgo where the well developed protein grains appear very 

 early in the egg cell. 



') Fujii, K., ..Kleinere Beiträge z. Mikrotechoik". „An. d. Glaskapillar 

 z. mikrochem. Analyse". (Compte Bendu d. sean. d. 6 Congres intern, d. Zool. 

 (Berne 1904). Geneve 1905. p. 531.) 



2 ) Bacibnrski, M., „Ein Inhaltskörper d. Leptoms". (Berichte d. D. Bot. 

 Ges. Bd. XVI. 1898. p. 52—63.) „Weitere Mitteil. ü. d. Leptomin"'. (loc. 

 cit. p. 119 and 123.) „Einige Demonstrationsversuche mit Leptomin". (Flora. 

 Bd. 85. 1898. p. 362' to 367.) 



