Stop es and Fujii, The nutritive relations of the surrounding tissues etc. 7 



If we follow the history of the starch cleposition in an older 

 series of ovules, for example in a series of Zamia ßoridana. We 

 find the following course of events. The first deposition of starch 

 takes place in the cells of the endosperm jnst at the hase of the 

 Archegonia, this spreads up the sides of the Archegonia leaving 

 the jacket cells empty, but soon appearing in the neck cells where 

 some grains are almost always fonnd np tili the time of fertilization. 

 In the course of the following month the aniount of starch deposited 

 in the endosperm cells steadily increases in quantity and in the 

 size of the grains. In the jacket, the cells at the base of the 

 archegonium are the first to be filled with starch grains and 

 gradually nearly all the cells of the jacket layer become packed. 

 A little later this starch is again dissolved away while yet the egg 

 cell and endosperm cells are well filled with grains (cf. fig. 5), 1 ). 

 [n the egg cell the starch appeared at first almost entirely at the 

 perifery, and this betöre they are deposited in the jacket layer. 

 These grains vary somewhat in size but are very much smaller 

 than those in the endosperm cells, only in one exceptional case 

 were they equally large. 



In egg cells about 2.6 to 3 mm long, the small starch grains 

 frequently appeared to be associated with protein grains which in this 

 stage were not deposited in the cells jnst near the egg cell but 

 were present in large quantities in those somewhat removed from 

 it. In further developed eggs about this size the appearance of 

 the starch and protein grains was very striking. In the egg cell 

 itself were present both starch and protein, the starch in very 

 minute grains, the protein substance being deposited in rather 

 irregulär, sometimes considerably complicated masses of very 

 various sizes. There were very few or no protein granules in the 

 jacket cells, and little in those cells of the endosperm adjoining 

 the jacket layer. In the rest of the endosperm, the cells contained 

 large quantities of protein, many of the grains being large and 

 irregulär exactly like those of the egg cell. In the cells further 

 removed from the egg cell the granules tended to become finer 

 and finer, tili the region was reached in which the protein appeared 

 to have filled the bulk of the cell in a viscous or semifluid 

 condition which in the fixed material shewed Spaces of bubble like 

 appearance. Tims the deposition of protein grains began first in 

 the cells near the egg, and in the egg itself as is also always the 

 case with starch. In later stages the cells nearer the egg cell 

 appeared empty of protein grains (cf. figs 7 and 8 of the same 

 thing in Ginkgo) which had been re-dissolved for the use of the 

 growing egg. 



A similar arrangement was seen in the case of the starch. In the 

 egg cell itself there were large nnmbers of extremely fine roundish 



v ) It is interesting to note that such as stage had been figured by 

 Warming in Ceratozamia robustä, though he did not attach any importancc 

 to it. Kesume. ..Rech. et. rem. s. 1. Cycadees". Tab. II. fig. 15. (Overs. d. Kon. 

 Danske Vid. Selsk. 1877.) 



