Stop es and Fujii, The nutritive relations of the surrounding tissues etc. 23 



10. So far as we coulcl see, the granulär contents of the 

 nutritive vacuoles have no nuclein among their constituents as in 

 most cases they do not stain with acetic rnethyl green. 



11. The chemical nature of the nucleoli and protein grains 

 is founcl to he different, as shewn by the results of digestion 

 experiments, supporting our view of their different origin. 



12. It is proved that the „Hofmeister Körpercheir' are not 

 nuclei, and we think it unlikely they are intimately connected with 

 nuclei. We suggest tliat they may be digestive vacuoles comparable 

 in origin and function with the digestive vacuoles of lower organisms, 

 which are forrned as required round the temporarily deposited food 

 in the egg cytoplasm. 



In the course of these investigations we have worked in the 

 botanical laboratories of the Universities of Manchester, London 

 and München, and the alpine Laboratory of Mount Schachen; and 

 we cannot express too strongly our best thanks to Professors Weiss, 

 Oliver, and Goebel for innumerable helpful kindnesses. We are 

 also indebted to many people for material which has been invaluable: 

 in particular we must thank M r Moore of Dublin, Prof. Chamberlain 

 of Chicago, Dr. Miyake of Kyoto and Drs. Yabe and Hattori 

 of Tokyo. 



Manchester and Tokyo. 



Explanation of plate. 



All figures drawn with the aid of a Camera lucida. For highest magni- 

 fication Beck's ^ju^h in oil immersion and Zeiss compens. ocular 12 were used. 



Fig. 1. EncepJialartos Lehmanni. Portion of the thick pitted wall between 

 egg cell and jacket cells, shewing final pit closing lamella perforated 

 only by Plasmodesmen. From band section swollen with sulphuric acid. 

 X 2200. 



w thickened wall. 

 I lamella closing pit perforated by groups of Plasmodesmen, cf. 



arrows a diag. 4. 

 j contents of jacket cells. 

 e contents of egg cell. 



Fig. 2. Encephalartos Lehmanni. Same as fig. 1. Shewing Plasmodesmen 

 through the thicker portions of the pit closing membrane. 

 t thicker portion of membrane cf. arrows b diag. 4. 



Fig. 8. Zamia muricatu. Surface view of a small portion of the thick wall 

 between egg and jacket cells shewing groups of pitted areas. From 

 microtome section tangential to egg cell. Staiued triple. x 900. 

 g group of pits. cf. diag. 4. pit to l st degree. 

 to thickest portion of wall. cf. diag. 4. A. 

 iv' irregularly thickened portions. cf. diag. 4. B. 

 r radial walls of jacket cells. 



