146 



STATE BOARD OP AGRICULTURE. 



on ordinary, lactose, and whey agar, while those micro-organisms asso- 

 ciated with the lactic prefer the ordinary agar. However, the results 

 are not especially marked in either case. In both instances, Miss Far- 

 rand Avas immediately responsible for the work. 



Another observation was made while conducting the butter studies. 



Miss Farrand, who did much of the bacteriological work in connection 

 with the butter studies, and practically did all the counting, did some 

 of the work in connection with the studies in association. It is singular 

 to note that she succeeded in securing more logical counts in butter 

 than in milk. The question may be asked: Was there less opportunity 

 for cells to group or adhere in butter than in milk, or taking a step 

 forward, was agglutination i)ossibly a factor in milk and not in butter? 



The sj'Stematic work conducted by Miss Is^orthrup for the committee 

 has some of the same features to deal with as have been considered 

 under the foregoing heads. Her work consisted largely of studying the 

 influence of agar of different compositions upon the determination of 

 the germ content at two temperatures, 21°C. and 3Ti4°C. Acidity, 

 lactose and peptone were the ingredients subject to variation. A sum- 

 mar^^ of her results are conveyed in the following table. 



» 



FINAL SITMMARY OF TEMPERATURE. 



Percent of samples growing best at 21°C. 63% 



Percent of samples growing best at .37.''C. 9% 



Indeterminate 28% 



FINAL Sr:MMARY OF ACIPITY, LACTOSE AND PEPTONE MEDIA, 



Temperature. 



21°C. 



37°C. 



Acidity 



Per cent of Inctosp. 



Per cent of Peptone — First lot . 



Per cent of peptone — Second lot . 



5° 

 10° 

 15° 

 20° 



0% 



2% 

 3% 

 4% 



0% 



1% 

 3% 



0% 

 1% 

 3% 



5% 

 21% 

 64% 

 10% 



15% 

 27% 

 22% 

 15% 

 21% 



22% 



6% 



72% 



35% 



38% 

 27% 



13 % 

 17 % 

 33 % 

 17 % 



20 % 

 10% 

 17i% 

 17i% 

 35% 



27 % 



6§% 



66f% 



35 % 

 23 % 

 42 % 



The comments I wish to make upon the detailed work of Miss North- 

 rup are these: 



1. The multitude of counts made b}' her is massive but open to 

 analytical criticism. The}' are irregular and seemingly illogical. Since 

 we have discussed this matter previously, we shall pass it over without 

 further remarks. 



