THE ALUMNI JOURNAL 43 



Ash Insoluble in Acid (Sand). 



Prepare total ash as above, boil with 25 cc. of 10^ HCl. for five 

 minutes, collect on gooch crucible, wash with hot water, ignite and 



weigh. 



Volatile and Non-Volatile Ether Extracts. 



Extract 2 grammes of the ground sample for 15 hours in a con- 

 timious extraction apparatus, with absolute ether; transfer the 

 ether solution to a tared capsule, or alumina milk dish, and allow to 

 evaporate at room temperature. Let stand for eighteen hours 

 over H_. SO- and weigh as total ether extract. 



Heat the extract gradually and then to no C. until weight be- 

 comes constant ; the loss is volatile oil, the residue non-volatile 



ether extract. 



Crude Fiber. 



Extract 2 grammes of the sample with ether or use the residue 

 for the determination of ether extract; to this add 200 cc. of 1.25% 

 boiling sulphuric acid ; connect flask with inverted condenser and 

 boil for 30 minutes ; a blast of air conducted into flask may serve 

 to reduce frothing of the liquid. Filter through linen or glass wool, 

 wash with boiling water until washings are free from acid, rinse 

 the substance back into flask with 200 cc. 1.25% Sodium Hydroxide 

 free, or nearly so from carbonates. Boil at once for thirty minutes 

 in same manner as above. Filter rapidly on gooch, wash with 

 boiling water until washings are neutral; dry at 110° C. until it 

 ceases to lose weight; weigh, incinerate completely and again 

 weigh. The loss is figured as crude fibre. 



OLD ALUMNI JOURNALS. 



Copies of the following numbers of the Alumni Journal are re- 

 quired to complete our files : 



Volume X, No. 3, 1903. 



Volume XI, all nund:»ers, 1904. 



Volume XII, all numbers, 1904. 



Volume XIII, all numbers, 1905. 



Any member possessing these copies, and not requiring same 

 for his files, will confer a favor by communicating with the under- 

 signed. Payment for the numbers will be made if desired. 



GEORGE C. DIEKMAN, M. D., 



No. 115 West 68th St., 



New York City. 



