34 



BOTANICAL GAZETTE [july 



thick "linin" bands; pair of chromosomes detached earlier lies at side of nucleus; 

 n, small nucleolus; nucleus cut. 



Fig. 25. — Spirem more or less completely segmented into chromosomes while 

 still in the second contraction stage; preparation considerably destained; 13 

 chromosomes in view. 



Fig. 26. — Spirem segmented, showing chain of eight chromosomes and three 

 pairs; nucleus uncut. 



Fig. 27. — Chain of six chromosomes, and probably four pairs; linin con- 

 nections between members of a pair not always visible; nucleus uncut. 



Fig. 28. — Fourteen chromosomes; several small nucleoli; nucleus uncut. 



Fig. 29. — Fourteen chromosomes, including five pairs more or less closely 

 associated; linin connections not visible; one pair of chromosomes has already 

 contracted into the globular shape. 



Fig. 30. — Fourteen chromosomes, several in pairs; apparent inequalities in 

 size due to positions in which some of the chromosomes are lying. 



Fig. 31. — Slightly later stage; the fourteen chromosomes contracted into the 

 globular or pear-shaped definitive form; linin connections longer and extremely 

 delicate; nucleus uncut. 



Figs. 32-34. — Other groups in diakinesis, showing various peculiarities of 

 chromosomes. 



Fig. 35. — Peculiar case of spindle formation; three nucleoli present and four- 

 teen chromosomes, including three or four pairs. 



Fig. 36. — Multipolar stage of heterotypic spindle; two more or less closely 

 united pairs of chromosomes present. 



PLATE III 



Fig. 37. — Same as fig. 36; an unusual case in which all the chromosomes 

 are closely joined in pairs; seven such pairs present and a small nucleolus. 



Fig. 38. — Heterotypic spindle in metaphase; spindle has usually more mantle 

 fibers than in O. Lamarckiana; chromosomes usually loosely arranged in equatorial 

 region of spindle. 



Fig. 39. — Late anaphase; an uncommon case; daughter chromosomes have 

 failed to divide, and fibrillae are scattered in cytoplasm at side of cell; chromatic 

 staining material also present. 



Fig. 40. — Telophase of heterotypic mitosis; exceptional case, in which a 

 rather sharp pointed spindle is formed at side of cell; it probably originated from 

 the fibrillae shown in Jig. 39. 



Fig. 41. — Early anaphase of homotypic mitosis; small nucleolus having the 

 characteristic appearance, present on one of the spindles. 



Fig. 42. — The single case of extra nuclei observed in O. rubrinervis pollen 

 mother cells. 



Figs. 43, 44. — Nuclei from telophase of second mitosis, passing into resting 

 condition. 



Figs. 45, 46. — Protoplasmic connections between mother cells. 



