212 Pennington — A Chemico-Pliysiological 



An effort to determine the individual acids of the mixture 

 failed because of the small quantity of material available, 

 but succinic acid could not be found, and tartaric acid 

 yielded its characteristic reactions. 



The sugars were determined by the reduction of Fehl- 

 ing's solution and the electrolytic estimation of the copper. 

 Starch was hydrolyzed and determined in like manner. 

 The sugar yielded 4.8 per cent., the starch 10.7 per cent, of 

 the material taken. 



The substance insoluble in hydrochloric acid was, when 

 dry, a perfectly white, homogeneous powder. It was 

 accepted as cellulose and the insoluble portions of the plant 

 ash, and upon weighing was found to equal 9.76 per cent. 



A Kjeldahl nitrogen determination, conducted according 

 to Gunning's modification of the method, gave a nitrogen 

 content of 2.61 per cent. Calculating the albumenoids on 

 this basis we have 16.31 per cent, of such compounds. 



The ash content of the water-free plant is 15.43 per cent. 

 If this be included in the analysis of the dry material we 

 have : 



Resin 3-° 2 



Glycerol 0.43 



Coloring matter • 2.62 



Tannin 8.71 



Mucilage 14-7° 



Gums 7- 2 4 



Acids 3.01 



Starch • 10.7 



Sugar 4-8 



Cellulose . . 9-7 6 



Albumenoids 16.31 



Ash 15-43 



Total 9 6 -73 



It is greatly to be regretted that the material could not 

 be obtained in pure culture in sufficient quantity to permit 

 of an investigation into the exact nature of the individual 

 substances of which it was composed. 



