124 C. U. C. P. ALUMNI JOURNAL August, 1918 



Regulation No. 3 — Specimens to be Numbered. Every specimen re- 

 ceived at the laboratory for the purpose of determining the presence of 

 communicable disease shall be numbered and so designated as to definitely 

 establish the identity of each particular specimen. 



Regulation No. 4 — Register to be Kepi\ The person in charge shall 

 cause a register to be kept v^^herein shall be entered the following intor- 

 mation : 



a — The laboratory number and date of the receipt of every speci- 

 men to be tested to determine the presence of a communicable 

 disease. 



b — The name of the physician submitting the specimen. 



c — The result of the laboratory test. 



d — The name of the person to whom the report of the result of the 

 test was forwarded. 



Regulation No. -5 — Equipment. The laboratory shall be equipped 

 with adequate facilities to properly perform such tests of specimens as 

 the laboratory undertakes to make. 



Regulation No. 6 — Methods. The methods employed shall be such 

 as are generally recognized as effective. 



Under regulations "5 — Equipment" and "6 — Methods," the following is given 

 by the department in collaboration with Dr. Poor, as being the minimum re- 

 quired. While it must needs be far beyond the capabilities of the average type, 

 it will demand however careful execution on the part of the more experienced. 

 For the following I am indebted to Dr. H. G. MacAdam, Chief of Division of 

 Institutional Inspection, Department of Health, City of New York : 



APPARATUS FOR MAKING THE FOLLOWING DIAGNOSIS 

 OF INFECTIOUS CONDITIONS 



1st — Examination of Sputum for Tubercle Bacilli. 



Platinum loop, glass slides, carbol fuchsin (decolorizer pref- 

 erably 3% HCl in alcohol and counter stain with methylene blue) 

 or Gabbets solution. Microscope, set of lenses including 1/12 oil 

 immersion or more powerful lens. 

 2nd — Diphtheria Cultures. 



Incubator maintained at approximately 37 degrees C, plati- 

 num loop, glass slides, Loeffler's methylene blue stain or equivalent, 

 e.g., Neisser's stain, microscope. 

 3d — Malaria. 



One of the blood stains, e.g., Jenner's, Wright's, Hasting's, 

 Goldhorn's, Giemsa's and microscope. 

 4th — E.vai)iinalion for Gonococcus. 



Gram's stain including aniline, or carbol gentian-violet. Gram's 

 iodine solution, alcohol and a counter stain. Microscope. 

 Sth—Widal Test. 



For the microscope test, a live culture of typhoid bacilli in 

 broth. Hollow ground slides with cover glasses. Microscope with 

 high power dry lens No. 6 or No. 7 may be used. An oil immersion 

 1/12 may he used. For the macroscopic test a dead culture of ty- 

 phoid bacilli. Test tubes. 

 6th — Spirochactac Pallida. 



Giemsa's stain or India Ink. Microscope or microscope with 

 dark field illumination attachment. 

 7th — Complement Fi.vation Wassermann Tests. 



Amboceptor, Antigen, Complement from guinea pigs. Sheep 

 cells 5% suspension. Graduated pipettes. Water bath. lest 

 tubes. Complement Fixation for Gonorrhoea — as above, plus gono- 

 coccus antigen. Complement Fixation for Glanders — as above, plus 

 glanders antigen. 

 8th — Differentiation Types of Pneumococcus. 



Mice, centrifuge, pipettes, antisera, test tubes, small syringe. 

 9th — Examination of Feces for Typhoid. 



Petri dishes, slides, etc. Antisera. Endo-Conradi or Teague 

 media. 

 10th — Examination of Blood for Typhoid. ^ 



Broth or bile flasks. ] 



