376 PHARMACEUTICAL BOTANY 
Sub-Class IV. Deuteromycetes or Imperfect Fungi—fungi 
whose life histories are incompletely known, as celery-blight, 
trichophyton, monilia, oidium, etc. 
THE PHYCOMYCETES, OR ALGA-LIKE FUNGI 
The Phycomycetes represent a small group of fungi showing 
close affinity with the green algee. ‘Their mycelium is composed 
of hyphz which are usually unicellular up until the time of 
reproduction and many-nucleated. This suggests a close rela- 
tion with the Spihonales group of green alge. ‘Their sexual 
organs are likewise similar in structure. ‘Transverse septa 
appear in the hyphe upon the formation of reproductive organs 
which separate these structures from the vegetative hyphz. 
They reproduce by spores as well as by sexual reproduction. 
Those Phycomycetes in which sexual reproduction is brought 
about through the union of like gametes belong to the order 
called <ygomycetales which includes the black bread molds and 
their kin; those in which sexual reproduction takes place through 
the union of unlike gametes belong to the Odmycetales or order of 
the watermolds and their allies. 
CuttivaTion or Motps.—Molds and other fungi will grow 
on most bacteriological culture media but do better if some sugar 
is added. ‘They may also be grown on moistened slices of bread, 
on slices of potato or carrot, or on decoctions of prunes, potatoes, 
manures, peas, beans, etc. Beerwort is also a good substratum 
for their growth. The liquid media may be made solid by the 
addition of agar. An excellent medium for the growth of molds 
and most other fungi in the laboratory is a modification of 
Czapek’s medium, the formula for which follows: 
BREN Se 1G iathes be ieee Pie eee a. ee 30.0 Gm 
Sethu Nitrates sent ia t  e  e  ee 20 
Dibasic Potassium Phosphate....-................ 1.0 
Magnesium Sulfate Crystals. os cc. ccc. 0.5 
pumennin CRORGG oe ee 0.5 
Ferrous Sulfate 
ya gs aay baie s Gen te ee 0.01 
WER ak ei Pc oe eek ee 1000.0 cc 
For solid medium, add Ager... 60 15.0 Gm 
Slide cultures are of great value in the study of molds since 
they permit the study of the aerial and submerged hyphae 
