APPENDIX II 681 
using. ‘This reagent imparts a red color to fixed oils but may 
stain other contents as well. 
AmMoniéA WATER.—An aqueous solution of gaseous ammonia 
containing in each 100 cc. not less than 9 Gm. and not more 
than 10 Gm. of NH;. Useful in clearing highly colored vege- 
table powders, such as ground roasted coffee, when mixed with 
equal parts of peroxide of hydrogen. Material should be 
macerated in this mixture within a tightly corked vial over 
night or longer and washed with water before examination. 
AMMONIA WATER, STRONGER.—This is an aqueous solution 
of NH; containing not less than 27 per cent. nor more than 
29 per cent. by weight of ammonia gas. It is employed in the 
preparation of ammonia water and cuoxam. 
ANILINE CHLORIDE.—A saturated aqueous solution acidified 
with hydrochloric acid is useful in staining lignified walls which 
are colored a golden yellow. : 
ANILINE SULFATE.—A saturated aqueous solution is em- © 
ployed as a test for lignified walls. Sections are mounted in . 
this reagent and a drop of sulfuric acid added. A yellow color 
is imparted to lignified walls. 
Anne BuLue.—A saturated aqueous solution is useful in 
staining sieve tubes. Sections should be placed in this solution 
for 24 hours and then washed to remove excess of stain. A one 
per cent. solution in 90 per cent. alcohol is used in double-stain- 
ing with safranin. It stains cellulose walls blue. 
Bismark Brown (ANILINE Brown).—A saturated aqueous 
solution is useful in double staining with gentian violet. A dilute 
aqueous solution made by dissolving 0.2 Gm. of Bismark Brown 
in 100 cc. of distilled water is of value in staining transparent 
tissues. 
CutLorAL Hyprate.—A solution prepared by dissolving 25 
Gm. in 10 cc. of water is an excellent clearing agent. It dis- 
solves starch, resin, protein, chlorophyll, etc., and causes more 
ess expansion of shrunken cells. ; 
Cutorav IopinE.—Saturate the previously prepared chloral 
hydrate solution with iodine by adding crystals of resublimed 
iodine to it and shaking. Useful for the detection of minute 
traces of starch or small starch grains, which are colored blue. 
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