682 PHARMACEUTICAL BOTANY 
CHLORZINCIODINE SOLUTION.— Dissolve 25 Gm. of anhydrous 
zinc chloride and 8 Gm. of potassium iodide in 8.5 Gm. of water 
and add iodine crystals to saturation. Keep in dark colored 
containers. Useful in distinguishing between cellulose and 
lignified walls. Cellulose walls are colored blue or violet and 
lignified walls yellow when mounted in this solution. It causes 
starch grains to swell and colors them blue. 
Curomic Acip SoLution.—Dissolve 5 Gm. of chromic acid 
in 45 cc. of diluted sulfuric acid (U.S.P.). A valuable reagent 
for separating the component cells in sections of plant organs. 
The sections are placed in this solution in a watch-glass and 
removed one by one, as required, in the course of 10 to 15 
minutes to a slide, washed with a few drops of water and sub- 
jected to pressure with a glass rod. 
CorRALLIN SODA SoLuTion.— Dissolve 15 Gm. of carbonate of 
soda in 35 Gm. of distilled water and add sufficient corallin to 
produce a pale bright pink color. This reagent stains the 
callus plates of sieve tubes pink. It should be freshly prepared 
as needed. 
Eosin.—An aqueous solution prepared by dissolving 1 Gm. 
of eosin in sufficient water to make 100 cc. is useful in the staining 
of cytoplasm, aleurone grains and other cell contents. If 
permanent mounts are desired, transfer to 1 per cent. acetic 
acid for a minute, wash with water, then to glycerin or through 
glycerin to glycerin jelly. 
A saturated alcoholic solution of eosin (using absolute alcohol 
as solvent) is employed when eosin-stained material is to be 
mounted in balsam. Acetic acid is omitted in this case and the 
sections passed from alcoholic eosin to a clearing agent as cedar 
oil or xylol, thence into balsam. 
ErHer.— Useful in defatting sections or powders of oil seeds. 
Fast GREEN.—A one per cent. solution in 95 per cent. alcohol 
is useful in staining tissue with cellulose walls green. 
FEHLING’s SoLuTION. (Alkaline Cupric Tartrate T.S.). 
Copper Solution.—Dissolve 34.66 Gm. of small, uneffloresced 
crystals of cupric sulfate in sufficient distilled water to make 
the mixture measure 500 cc. Keep in well stoppered bottles. 
