684 PHARMACEUTICAL BOTANY 
by dissolving 3 Gm. of Ferric Ammonium Sulfate in sufficient 
distilled water to make 100 cc. The stain (Solution II) is pre- 
pared by dissolving 0.5 Gm. of hematoxylin in 100 cc. of distilled 
water. The mordant and the stain are used separately on the 
sections or tissues to be treated. 
Hyprocutioric Acip.—The concentrated C.P. acid is used 
with an equal volume of Phloroglucin Solution in determining 
lignification. Dilute solutions are employed for neutralizing 
alkaline solutions, etc. 
Iopine WAtTER.—Add as much iodine to distilled water as it 
will dissolve. Stains starch pale blue to purplish-blue. 
IopINE AND PorasstuM Iop1bE SoLution.—Dissolve 1 Gm. of 
iodine and 3 Gm. of potassium iodide in 50 cc. of water. Keep 
in glass stoppered bottles. This reagent stains cellulose, ligni- 
fied and corky walls and proteins yellow and starch blue to 
bluish-black. 
LABARRAQUE’S SOLUTION.—Triturate 50 Gm. of fresh chlor- 
inated lime with 250 cc. of water so as to form a uniform mix- 
ture. Dissolve 35 Gm. of monohydrated sodium carbonate in 
250 cc. of hot water, and add this solution to the chlorinated lime 
mixture in a suitable vessel. Stir or shake thoroughly. (Warm 
if solution gelatinizes.) ‘Transfer the mixture to a wetted muslin 
strainer, returning the first portion until the liquid passes through 
clear, and when no more liquid drains from it, wash the precipi- 
tate with sufficient water to make the product weigh 500 Gm. 
Keep in amber colored, glass stoppered bottles in cool place. 
It gradually loses in strength on standing. ‘This reagent is 
used for bleaching highly colored sections and powders. The 
materials bleached should always be washed with water before 
mounting. 
Licnut Green.—This is used as a combination stain with 
safranin. Dissolve 1 Gm. of light green in 100 cc. of clove oil; 
25 cc. of absolute alcohol and 75 cc. of clove oil may be used in 
place of pure clove oil, if the light green is found to precipitate: 
out when xylol is subsequently employed as a clearing agent. 
NaAPHTHOL SOLUTION.—Dissolve 5 Gm. of alpha-naphthol in 
50 cc. of alcohol. Used in conjunction with concentrated sul- 
furic acid for the detection of inulin. <A violet coloration is 
