690 PHARMACEUTICAL BOTANY 
7. Mount in Canada balsam. 
8. Label slide. 
The safranin will impart a red color to the suberized and lig- 
nified walls, while the light green will stain cellulose walls green. 
Should the light green precipitate upon clearing in the xylol, 
add 3 cc. of absolute alcohol to each 100 cc. of xylol. 
Should air-bubbles be detected in the balsam shortly after 
mounting, heat a dissection needle in a flame and touch each 
with its tip, when they will be found to disappear. 
If too much Canada balsam has been used, some of it usually 
spreads beyond the edge of the cover-glass, or on its surface. In 
this event wait until the balsam hardens, when it can be scratched 
off with a knife, and the surface of the glass cleaned with a rag 
moistened with turpentine oil or xylol. i 
Should the Canada balsam become too thick, it can be 
thinned down with either xylol or benzol. 
No. 3. SAFRANIN AND ANILIN BLUE 
1. Stain sections with either alcoholic or anilin safranin (1 per 
cent. sol. in 50 per cent. alcohol) for from 1 to 24 hours or by 
heating them in the staining fluid until vapors arise. 
2. Wash out excess of stain with 50 per cent. alcohol. Be 
careful not to remove too much of the safranin. Washing should 
stop as soon as the stain is reduced to a pink in cellulose walls. 
3. Stain with Anilin Blue (1 per cent. sol. in 90 per cent. 
alcohol) for from 3 to 10 minutes. 
4. Wash in 95 per cent. alcohol for a few seconds. 
5. Rinse with slightly acidulated 95 per cent. alcohol for a few 
seconds. 
6. Wash for 1 to 2 minutes in 95 per cent. alcohol containing a 
trace of an alkali to remove the acid. 
7. Dehydrate with absolute alcohol for two minutes. 
8. Clear in cedar oil for one minute. Blot up excess of cedar 
oil from around section. Clove oil or xylol may be substituted 
for the cedar oil. 
9. Mount in balsam. 
10. Label slide. 
If the sections are properly stained, the lignified and suberized 
walls will be stained red and the cellulose walls a brilliant blue. 
