CULTIVATION AND PRESERVATION OP PENICILLIA 



75 



claviformin are discussed in other portions of the text, these subjects need 

 not be considered here. 



Fig. 18. Spectrum-plaie metiiud for tne ideiuitication of antibiotic substances 

 produced by Penicillia. .1, Agar plate with trough containing solution of crystalline 

 citrinin, showing the effect of this antibiotic upon the growth of six test micro- 

 organisms streaked as follows from top to bottom of the plate: Proteus inirabilis, 

 XRRL B-400; Salmonella schottmuelleri, NRRL B-119; Staph ulocoecus afireiis, NRRL 

 B-313; Bacillus suhtilis. XRRL B-o58; Candida albicans, XRRL Y-477; and Bacillus 

 cereus, XRRL B-569. B, A plate similarly streaked four days after being inoculated 

 with Penicillium citrinum, XRRL 1842. " Xote the identical patterns of inhibition 

 in A and B. C, The same type of test in a four-day culture of a penicillin jiroducing 

 mold, P. chrysogenwn, NRRL 1951. B25. D, The same type of test in a four-day cul- 

 ture of a claviformin producing mold, P. claviforme, NRRL 1002. See discussion in 

 text, pp. 74-75. 



Micro-Cultures 



Ullseheck (1928) based his description of morphology upon cultures 

 grown upon minute quantities of agar in sUdes manufactured for hanging 

 drop studies. The culture chamber or depression was covered with a 



