214 A MANUAL OF THE PENICILLIA 



NRRL 2045, received in May 1946 from Professor W. H. Weston as an 

 isolate from deteriorating tentage in the Canal Zone, represents something 

 of an extreme in producing very restricted and exceedingly close-textured 

 colonies that are strongly raised and frequently split open in central areas; 

 penicilli are typically smaller and are borne upon very short conidiophores 

 arising from closely felted hyphae. This culture may be regarded as 

 approximating the one cited by Thom in his Monograph (1930, p. 199) as 

 strain number 4876.24, received originally from Westerdijk. 



NRRL 749 is representative of variation in another direction. Colonies 

 on Czapek are larger, up to 3.0 cm. in 2 weeks, comparatively thin, very 

 strongly wrinkled, with central areas commonly depressed and with sub- 

 central areas raised and often splitting from the extreme folding of the 

 mycelium, more or less zonate, rather heavy sporing in dull yellow to gray- 

 green shades, and with reverse yellow to drab but not in vinaceous or 

 greenish shades; the penicilli are characteristic of the species in origin and 

 in pattern. 



NRRL 2068 is representative of a group of very slow-growing strains 

 isolated from fabrics and other military equipment undergoing deteriora- 

 tion in tropical and subtropical areas. Colonies grow very restrictedly 

 on all media, and on Czapek, commonly show no evident growth for several 

 days after inoculation and reach a diameter of 1 cm. only after 10 to 14 

 days. Colonies are very close-textured, tough, with central areas un- 

 colored or pinkish, with exudate clear to light amber, and showing limited 

 conidial development only in submarginal areas. Colonies on malt agar 

 are similarly restricted but heavier sporing and in somewhat lighter yellow- 

 green shades than such typical strains as NRRL 746. The peniciUi are 

 typical of the species in origin and in pattern. 



Three additional species described by Biourge are believed to represent 

 little more than strain differences, hence to belong with his PenicilUum 

 fellutanum. The types upon which these species were based show limited 

 strain individuality, but are not believed to show sufficient differences one 

 from the other or from P. fellutanum to warrant their continued recogni- 

 tion. 



PenicilUum phaeo-jatithinellum Biourge (Monograph, La Cellule 33: fasc. 1, pp. 

 289-290; Col. PI. VIII and PI. XIII, fig. 77. 1923) as described and illustrated by 

 him, seems to approximate P. fellutanum. No authentic material has been available 

 for the present study, but Them's notes made prior to 1930 on Biourge's type (Thorn's 

 No. 4733.96) indicate a culture of the type discussed above, which apparently differed 

 from these only in being somewhat faster growing (4.0 cm. diam. in 2 weeks) and 

 possibly looser in texture. A strain received from the Centraalbureau in July 1946, 

 bearing this name, as an isolate from "Gambir" in 1927, shows limited strain individ- 

 uality but lacks essential differences to separate it from P. fellutanum Biourge as the 

 species is considered here. 



