ASYMMETRICA-DIVARICATA 297 



Centraalbureau and is now maii^itained by us as NRRL 2025. This latter 

 culture no longer produces colonies conforming with Zaleski's (1927) and 

 Thorn's (1930) descriptions, nor does it produce well-developed penicilli 

 on any culture medium emploj^ed. The conidia, however, are entirely 

 characteristic of the species. Due to their unique markings there is no 

 question as to the authenticity of the culture from Baarn. 



Since the culture now in our possession fails to present the cultural 

 aspect of the species as originally isolated and studied, the description pre- 

 sented here represents a condensation of Zaleski's original diagnosis as 

 presented by Thom in his Monograph (1930, p. 361), followed by pertinent 

 culture notes by the latter investigator made prior to 1930: 



"Colonies in neutral Raulin with 10 per cent gelatine in petri dishes, slowly growing 

 becoming 24 to 26 mm. in diameter in 12 days, liquefying the gelatine tardily but 

 completely, velvety or somewhat closely subfloccose, zonate only indistinctly and in 

 the outer area, with the whole central area thrown into broad regularly radiate 

 wrinkles, with the very center somewhat depressed and showing a few uncolored 

 drops; white marginal zone 2 to 3 mm. wide; in color conidial areas at first blue-green 

 shades such as 371, 372, ' becoming dark yellow-green shades such as 273, and later 

 dark orange-brown such as 168, 164, 198, 139; reverse at first in orange-yellows such 

 as 171, 166, 157, to 133, 138, later becoming red-orange 84, 88, 92, 97; odor none or 

 weak; conidiophores 10 to 200 or 300 by 2 to 2.5//, with apex more or less enlarged or 

 inflated, commonly unbranched, occasionally with short branches, flexuous, varying 

 greatly in length, erect or ascending; penicilli mostly 10 to 12yu, less frequently 25 to 

 30m or 40^1 long, with walls smooth; metulae 8, 10, to 20 or 24 by 2.5 to 3.0m, in groups of 

 2 or 3, commonly unequal and irregularly arranged, with apices commonly inflated; 

 sterigmata about 9 to 10 by 2.5 to 3.0^, commonly in verticils of 3, 5 to 10 or 12, some- 

 times occurring singly; conidia 2.5 to 4.0ai by 2.5 to 3.0^, varying considerably in size, 

 coarsely denticulate, ovate elongated or subglobose. 



"Habitat: Species isolated from soil under pine near Poznan, Poland." 



Thom's notes follow: 



"The type strain growing well at both 20° and 30°C. Colonies upon Czapek's 

 solution agar at 20°C., spreading fairly widely (30 mm. in diameter in seven days), 

 floccose with some funiculose or fasciculate hyphae (more deeply floccose in slanted 

 tubes than in petri dish cultures), gray -green with white marginal areas 2 to 3 mm. 

 in width during the growing period (at 30°C. pitted mycelial mass thinner, radiately 

 wrinkled and wanting in green color); reverse in areas purple drab (Ridgway, PI. 

 XLV) ; drops in central area, colorless; conidia coarsely roughened with winding color 

 bars, elliptical to subglobose 4 by 3/i." 



Our current notes on NRRL 2025 follow: Colonies on Czapek's solution 

 agar attaining a diameter of 4.0 to 4.5 cm. in 12 days to 2 weeks at room 

 temperature (24°C.), producing a rather delicate basal felt with surface 

 growth somewhat floccose and with marked development of funicles often 

 apparent at the colony margin, radially furrowed, almost azonate, white 



1 Color references refer to tabs in Kleincksieck and Valette's Code des Couleurs, 

 Paris. 1908. 



