308 THAXTER. 



Endogone fasciculata nov. sp. 



(Figs. 21-28.) 



Spore-masses spongy, loosely coherent, rather thin and irregularly 

 lobed, somewhat amorphous, 10-14 X 4-5 mm., but very variable, 

 incorporating more or less of the substratum (Sphagnum) and other 

 foreign matter. Chlamydospores in rounded or somewhat elongate 

 or irregular coherent groups, associated with less definitely distin- 

 guished masses of readily separable zygospores; pale yellowish or 

 faintly brownish, mostly spherical or somewhat longer than broad, 

 60 X 60-85 X 70 fx, the wall becoming relatively very thick, 6-10 fx. 

 Zygospores immature, irregularly spherical, colorless, about 50 n, 

 arising from the larger of two unequal gametes. 



In Sphagnum. Little Metis, P.Q. E. C. Jeffrey. 



This species is in some respects the most interesting member of the 

 genus, since it is not only peculiar from the grouping of its spores, but 

 presents the only instance in which zygospores and chlamydospores 

 have been found intimately associated in the same spore-mass. It 

 thus furnishes the first indubitable eA'idence that the zygosporic and 

 chlamydosporic types have been rightly included in a single genus. 



None of the zygospores examined are mature, but there is no indi- 

 cation that any special envelope is developed about them, as in E. 

 ladiflua and some other sexual forms; although the process of forma- 

 tion. Figures 23-26, is very similar to that which occurs in the last 

 mentioned species. The hyphae with which they are associated are 

 thin-walled, scanty and evanescent; so that even in the youngest 

 stages of conjugation, the exact origin and relation of the progametes 

 is not clearly evident. Although this cannot be regarded as deter- 

 mined beyond question, examination of young stages under an immer- 

 sion seems to show that the type of conjugation is homothallic, and 

 that the progametes arise in proximity to one another from the same 

 filament. The gametes are distinguished much as in E. ladiflua, one 

 being larger than the other, and bearing the zygospore, which bulges 

 upward; both remaining attached, with slightly thickened walls and 

 septa. The groups of zygospores are more irregular and undifferen- 

 tiated than those of the chlamydospores, among which they are 

 irregularly distributed in continuous masses. 



The chlamydospores arise from a plexus of clearly defined, thick- 

 walled, variously bent and interlaced branching hyphae, which form a 

 core from which short irregular sporiferous branches grow radially 



