116 Agriottltural Experiment Station, Ithaca, N. Y. 



day, transpiratioTi being greater, the leaves were somewhat flabby 

 though later in the day and during the night the}' were turgid.* By 

 the 8th two large cushions of turgescent tissue had formed on one of 

 the cuttings of the No. 18 variety, one on the stem about 2^ cm long 

 'and extending about one-fourth the distance around the stem; the other 

 one on the under side of a petiole about 3 otn long. At noon on the 

 8th the experiment was changed by placing a glass (age over the plants 

 to lessen transpiration. The plants and the inside of the cage soon 

 became so wet that water streamed slowly down. The cage remained 

 on until the 10th. During this time the plants remained so wet no 

 observations could be made on the development of the cushions. 



During the preceding night the tissue of the No. 18 variety, being 

 much more tender than the others, collapsed where the rubber tube 

 was wired to the stem and permitted Avater to spurt through the 

 delivery tube over the other plants. The cage was then removed at 

 8:45 A. M., and two plants were replaced with fresh ones, those being 

 removed were the No. 18 variety and the plant the leaves of which 

 had partially dried at the time the experiment was set up. Both of 

 the fresh plants introduced were of the Lorillard variety. At the 

 time of the mid-day observations on the 10th it was noted that the two 

 other plants which had been connected four days, two days of the time 

 under the moist cage, were drooped. They were left for another day 

 with the hope that lessened transpiration during the night might 

 revive them. On both of these plants, which were of the Lorillard 

 variety, quite extended cushions were developed on the stem. On one 

 plant were two cushions each about 3 cm long, on the other was one 

 cushion 2^ cm long. The elevated, shining mass of cells could readily 

 be seen so soon as they had dried after removal of the cage . Decem- 

 ber 11th these two plants were still drooped,' probably not having 

 revived during the night. They must have been near dissolution, for 

 some of the leaves easily fell away from the stem. While there 

 probably had been sufficient pressure on dui'ing the past 24 hours, the 

 faucet was but a little way open and much iron rust had formed and 

 clogged the vascular ducts at the point of union with the delivery 

 tubes so that one of the fresh plants introduced the previous day had 

 drooped. The three drooping })lants were then replaced with fresh 

 ones, one cutting being of the No. 18 variety and possessing quite a 

 firm stem at the base. The water was turned on full pressure.! It 



*It was latter found that iron rust accumulated slowly in the larger rubber 

 tubes and partially clogged the vascular ducts, which accounted for the flabby 

 condition during the middle of the day. 



fThe pressure of the water was about 20 to 80 lbs. 



