286 Bulletin 178. 



Bacteriologic Examination of the Glandular Tissue. 



Groiijp 1. — -In this examination* a large number of snb-cnltnres 

 were made. The growth of the cultures was studied on several 

 kinds of media and eacli was examined microscopically. They were 

 found to be nearly all micrococci and to belong to one of three 

 species. A spore bearing bacillus belonging to the Bacillus suhtilis 

 group and another organism probably B. fluorescens liquefaciens 

 were occasionally encountered in this and the following examina- 

 tions. It is possible that they are contaminations, but our lack of 

 knowledge of the bacteria in freshly drawn milk renders it unsafe 

 to deny their presence within the udder. A number of other cul- 

 tures, each found in but a single instance, were discarded. The 

 writer does not wish to be understood as offering the following 

 brief summaries of cultural characters as full descriptions. The 

 loss of most of the cultures has rendered it impossible to present 

 but little more information concerning their cultural characteristics 

 than was considered in classifying them into the three groups of 

 similar cultures. 



Micrococcus No. 1. 



Morphology. — A micrococcus about l/^t in diameter. 



Staining. — Stained readily by the common anilin dyes. 



Agar. — The growthf is white, shiny and viscid rather than 

 friable. 



Alkaline houillon. — This medium becomes slightly clouded with 

 the deposition of a white sediment easily disseminated by agitation. 

 The reaction remains constantly alkaline. 



Ten per cent gelatin, stab. — Colonies are white and are very 

 slightly depressed, due apparently to a slight liquefaction. In a 

 stab culture the needle's j^ath is marked by a whitish growth. The 

 surface exhibits a white colony of creamy consistency, but no gene- 

 ral liquefaction occurs even after a month's growth. 



*The writer is indebted to Dr. Cooper Curtice for the privilege of examining 

 the above group of udders. 



f The temperature, except in the case of gelatin cultures is to be understood 

 as 37.5 degrees centigrade. 



