Invasion of the Udder by Bacteria. 



2S1 



Ferinentation tribe. — Growth in one per cent solutions of glucose 

 and of lactose in bouillon occurs only in the open arm of the tube. 

 The reaction remains alkaline. 



Milk. — Litmus milk remains blue and is not visibly changed. 



TABLE No. I. 

 The Source of Pure Cultures of Micrococcus l^o. 1. 



Micrococcus T^o. 2. 



Morphology. — A micrococcus about l,a in diameter. 



Staining. — Stained readily by the connnon anilin dyes. 



Agar. — Color"^ varies from cream white to ocher depending upon 

 age and other undetermined conditions. During active growth the 

 color assumes successively the tints lying between cream white and 

 ocher. The color is the distinguishing feature of the growth upon 

 agar. The color of old cultures fades. 



Alkaline houillon. — Growth renders the liquid clouded, with the 

 deposition of a yellowish sediment. Keaction remains constantly 

 alkaline. 



Milk. — Milk is coagulated in from three to five days. 



Ten per cent gelatin^ stab. — Tliis medium is gradually liquefied, 

 the fluid becoming turbid, while a yellowish sediment is deposited. 

 The liquefaction extends downward in a zone involving the whole 

 area of the tube, but preceded slightly by a pit following the track 

 of the inoculating needle. 



Fermentation tribe. — Growth in one per cent solutions of glucose 

 and lactose in bouillon, occurs only in the open arm of the tube. 

 The reaction remains alkaline. 



* The writer employs Saccardo's color chart for the nomenclature of colors. 



