APPENDIX. 227 



were again examined by Mr. Buckland, Mr. Mar- 

 croft Jolinson, myself, and other gentlemen who 

 take an interest in the experiment, and the ova 

 found to be in perfect health. They had been 

 fifty-nine days in the refrigerator, and the ice-box ; 

 at a temperature of 34° in the former, and 33° in 

 the latter. A few were taken out and given to Mr. 

 Buckland and Mr. Johnson to test their vitality by 

 hatching them in the usual way. — March 28th. 



" After ha\T.ng replaced these boxes, we went to 

 the ice-vaults of the \Yenham Lake Ice Company, 

 at Blackfriars, and exhumed one of the boxes con- 

 taining ova that had been buried (as many persons 

 prognosticated, in their icy graves) on the 17th of 

 January last. I cannot describe the anxiety of all 

 present to get a first sight of these ova, or the 

 pleasure "sdsible on every countenance to find as the 

 moss (shrouds, as many expected) was removed, our 

 little friends alive and perfectly healthy. Mutual 

 congratulations and shaking of hands ensued. Mag- 

 nifying glasses were produced, and the ova most 

 carefully examined, to ascertain if our first impres- 

 sions were true. ^ All right ! ' exclaimed the in- 

 defatigable Buckland, ' all right ! ' 



"Some of the ova were then given to Mr. 

 Johnson, and the exhumed box with the re- 

 mainder carried ofi" in triumph by Mr. Buckland, 

 to test their vitality by endeavouring to hatch 

 them. 



" Some small pieces of ice which I had placed 

 on the top of the moss inside the box, with the 

 idea that they would melt, and thus give a small 

 supply of water to the ova, were found in the same 

 state as when deposited — a proof that the tempera- 



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