crystals even after standing for 7 days. Absolute alcohol was tlioii added to tlie 

 synip to remove matters solnl)le in the alcohol, well mixod and decanted. The 

 insolnbe residue in absolute alcohol was dried in vacuum over sulphuric acid, 

 and its specific rotatory power determined. The following result was found : 



(«)D= 0-4xO.B4GxlO ^ + 92.27 (at 20°). 

 ^ ^ 0.015 X 1 ^ ^ 



The residue was again treated witli methyl- and absolute alcohol to remove 

 the accompanj^ing matter, in exactly the same manner as above described. The 

 substance here obtained was almost tasteless. The specific rotatory power was 

 determined and found to be 4- 103.8. 



(«) D = Q-45 X 0.346 X 10 ^ ^ ,^^ 20° ) . 



^ ^ 0.015 X 1 ^ ^ 



The residue was once more purified in the same manner as in the above 

 cases. The specific rotatory j^wwer was determined and found to Ije identical 

 with that of the above case. 



(a)D= 0^3 X 0.346 xK)_^ +103.8° (at 20°). 

 ^ ^ 0.01 X 1 ^ 



Next, we determined the quantity of mucic acid which is produced from the 

 residue by oxidation. 0.0834 gi-am of the residue and niti-ic acid of sp. gr. 1.15 

 were mixed in a small lieaker and oxidized in a boiling water bath according to 

 the usual method. The mucic acid produced was collected on a filter and weighed 

 0.0065 gram corresponding to 7.79^. 



According to Tollens^\ the sx)ecific rotatory power of raffinose is +103.0° or 

 + 104.0° wliile Lippmann'> found it to be +105.0^ and -\-104t 95°. The producticm 

 of mucic acid from 0.1 and 0.075 gi-am raflinose according to Creydt^^ is 0.009 

 and 0.0056 gi-am coiTesponding to 9.0^ and 7.5% respectively. 



Consequently, the residue under examination would x>robably be raffinose. 



6. Exiyeriiiient tvith residue {II)' 



The residue, insoluble in lx)iling 95^ alcohol, was slightly dark coloured 

 and had a slightly sweet taste. Upon qualitative tests, the residue gave reactions 

 almost similar to those of residue (III). 



1) ToUens,- Zs. d. Vcr. d. D. Zuckerind., Bd., 35, P. 31 and 591 ; Lippmann,- Chemie d. Znck- 

 erarten, Braunschweig, IM. IT, P. 163G (1904). 



2) Lippmann,- ibid., Bd. 35, P. 257; Bd. 38. P. 1232; Lippmann,- ibid., PP. 1636- 1637. 



3) Creydt,- I.e. 



