Hemmi : On a Disease of some Leguminous Plants caused by CcratopJionun setosiun Kirchner i 2 3 



seem to be originated by the more or less dense formation of short lateral irregu- 

 lar branches composed generally of a i^w chlamydospores which adhere firmly to 

 each other forming irregular dark masses. The single chlamydospores are glo- 

 bose, subglobose or ellipsoidal in shape, or they become often more or less angular 

 in shape by the mutual pressure of the adjoining cells in a knot. The chlamydo- 

 spore-formation of the fungus was first noticed by Rostrup(8) in 1905, and Lind(4) 

 reproduced two of the Rostrup's figures in his Danish Fungi in 1913. 



8. Inoculation Experiments 



The inoculation experiments, from which the conclusions of the parasitism of 

 the fungus and also the identity of the fungus which attacks Lupinus polyphylbis^ 

 Cytisus capitatiis and one other species of the same genus have been drawn, were 

 made in the laboratory of our institution in the month of July of this year. 



Experiment I. 



On July 4, the first inoculation test was made on the healthy leaves of the 

 seedlings of Cytisus capitatus, which were growing in a pot and kept on a labora- 

 tory table. Small bits of the mycelium and chlamydospores from corn-meal agar 

 cultures isolated from Lupi)ius polypJiyllus were placed by a sterile needle in drops 

 of water on both sides of the uninjured leaves, which were previously sprayed 

 with sterilized water. To keep it moist, we placed the pot under a bell-glass 

 covered inside by the moistened filter-paper for two days. 



All of the leaves which had been inoculated showed signs of infection at 

 about the fourth or fifth day. The disease did progress rather rapidly, forming 

 blackish brown spots on the leaves which gradually shrunk, dried up and at last 

 dropped to the ground. But all uninoculated leaves treated in the same way as 

 controls showed no changes for a long time. 



Experiment II. 



On July 6, the second inoculation test was made on the healthy young leaves 

 of Lupinus polyphyltus. When the plant was transplanted into a pot, all the old 

 leaves were cut off and the pot was kept ia tlie laboratory in order to avoid the 

 natural infection. 



Small bits of the mycelium and chlamydospores from corn-meal agar cul- 



