ttires isolated from a species of Cyiisus were placed by a sterile needle in the drops 

 of water on botli sides of the uninjured leaves, which were previously sprayed 

 with steiilized water. To keep it moist, we treated in the same way as in the 

 case of the first experiment. The inoculated leaves showed the sis^ns of infection 

 at about the third or fourth day as small peitches of brown spots. The diseased 

 leaflets wilted then gradually from the ends. In the case of this experiment, we 

 inoculated the fungus also on some healthy petioles in the same way. It showed 

 also the positive result at about the fifth day and at last the petioles were broken at 

 the infected points. Although the symptoms of the disease are rather different 

 from the naturally infected leaves, such a difference is undoubtedly due to the 

 wideness of the inoculated area and youngness of the leaf. The leaves treated in 

 the same way as controls showed no changes for a long time. 



Expetiment III & IV. 

 On July lO, the third and fourth inoculation tests were made on the healthy 

 leaves of Lupiiius polyphylhis grown in pots. In those cases, we used also bits of 

 the mycelium and chlamydospores from corn-meal agar cultures as the inoculum. 

 But in the case of the third test, the fungus isolated from Cytisus capitatiis was 

 used, and in the case of the fourth test, that isolated from Lnpiiius polyphylhis was 

 used as the inoculum. The methods of these inoculation experiments were quite 

 the same as those above described. The results of these two cases were nearly 

 the same. The signs of the disease could be detected on the leaf as brown patches 

 at about the fourth or fifth day and then the infected area shrunk gradually and 

 at last the leaflets wilted from the end. But the control-leaves treated in the same 

 way were all healthy for a long time. 



Judging from the results of the above experitnents, we may safely infer that 

 the causal fungus isolated from each of the three different hosts belongs all to one 

 species, and that it is a virulent parasite, easily infecting the uninjured leaves. It 

 is not always easy to explain how a parasite gains entrance into its host. It enters 

 always from the inoculated portion on the both sides of the uninjured leaves and 

 also on the healthy petioles. Although we have not been able to demonstrate 

 clearly whether the fungus makes the stomatal infection or the cuticular, we have 

 a conviction from what we have observed that the cuticular infection is taking 



