194 Hub er: Methud of preparing laigc Nurabers of Sections. XXIII, 2. 



tliis purpose rectaiigulai- glass trays, a little larger than tlie plates 

 used are convenieiit. The glass-plate with the sections adhering to 

 one side is now removed from the alcohol , i)laced on edge for a 

 few moments and is then covered on the section side with a thin 

 layer of the following photoxylin Solution : 



Photoxylin 10 g 



Absolute iilcohol 100 cc 



Ether 500 „ 



The photoxylin may be dissolved in about eqiial parts of alcohol 

 and ether and the ether not used added when Solution has been 

 obtained. Of this Solution a small quantity is poured on the section 

 side of the plate after this is taken from the absolute alcohol. The 

 plate is then slightly rocked so as to spread the photoxylin Solution 

 as evenly as possible. The excess is then drained from one angle 

 of the plate into the stock bottle of the photoxylin Solution. On the 

 evaporation of the ether and alcohol the photoxylin forms a thin 

 but strong film, to which, as will be seen, the sections adhere. As 

 soon as the film of photoxylin has formed, which may readily be 

 ascertaiued by inspection or by touching it with the finger , this is 

 cut with a Sharp knife along the borders of the plate, about 5 mm 

 from the edge , or by the cutter shown in fig. 2 , and the plate 

 placed into distilled water , when tlie film of photoxylin with the 

 adherent sections will looscn readily from the glass-plate. If a 

 number of plates are to be manipulated at the same time , a plate 

 may be carried to the alcohol, another to the xylol and another 

 placed on the warming plate and carried from one step to the other 

 as rapidly as the manipulations can be carried on; it being only 

 necessary not to allow the sections to dry after the plate has been 

 removed from the absolute alcohol, before coating the sections with 

 the photoxylin Solution and also, not to allow the film of photoxylin 

 to become too dry before placing the plate into distilled water. 

 Such films of photoxylin, measuring about 15 cm in length and about 

 10 cm in width may readily be made to contain 50 to 75 sections 

 of the size generally given out in regulär laboratory work. They 

 may be stained after any of the methods generally used. Mention. 

 may be made of the hematoxylin- eosin method or hematoxylin and 

 other of the acid anilin dyes used in connection with it; of hema- 

 toxylin and VAN Giesen's picric acid and fuchsin stains and the 

 manner of using this method as recommended by Weigert (9) has 



