196 Huber: Method of preparing large Numbers of Sections. XXIII, 2. 



bubbles which collect on the surface of tlie film. The tray is then 

 placed so tbat one of tbe narrow edges of the plate is toward the 

 manipulator and the film is loosened for a short distance from this 

 edge and applied to a glass-tube a little longer than the width of 

 the film, and about 5 mm to 8 mm in diameter and if the loosened 

 portion of the film has been evenly applied to sides of the glass- 

 tube , the remainder of the film may be readily loosened from the 

 glass-plate and roUed upon the tube as this is caused to rotate over 

 film and plate. Before placing the dried film into the distilled 

 water, this may be marked as desired with India ink, or a small 

 Strip of thin paper , bearing the necessary Information , written in 

 India ink may be placed on the glass-plate and included in the 

 film with the sections. The glass-rods with the photoxylin films 

 rolled on them may be stored in 80 ^/q alcohol in large tube vials 

 (18 cm high and 5 cm diameter). If it is desired to unroll a 

 photoxylin film from a glass - tube , this is placed in distilled water 

 when the film may be readily uurolled and stained as desired. 



To Mr. Clarence Snow , the assistant in the laboratory , avIio 

 has used this method for some time in the preparatiou of the class 

 material, I am indebted for tbe working out of many of the details 

 of the method as now used an here described. 



References to Literatiire. 



1) Weigert, Zeitschi-, f. wiss. Mikrosk. Bd. II, 1885, p. 490. 



2) 0BREC4IA, Neurolog. Zcntralbl. Jahrg. IX, 1890, p. 295. 



3) GuLLAND, Journ. Path. and Bact. vol. I, 1893, p. 391. 



4) Strasser, Zeitschr. f. wiss. Mikrosk. Bd. III, 1886, p. 346. 



5) Strasser, Zeitschr. f. wiss. Mikrosk. Bd. IX, 1892, p. 1. 



6) Strasser, Zeitschr. f. wiss. Mikrosk. Bd. XII, 1895, p. 154. 



7) Heidenhain, Zeitschr. f. wiss. Mikrosk. Bd. XXII, 1905, p. 330. 



8) Kolster, Zeitschr. f. wiss. Mikrosk. Bd. XVIII, 1901, p. 170. 



9) Weigert, Zeitschr. f. wiss. Mikrosk. Bd. XXI, 1904, p. 1. 



[Eingegangen am 8. Juni 1906.] 



