THE rilYSlULUGY OF I'UlSONING 211 



sins (staining phenomena), is met with again in the case of extracts 

 of organs, at least with regard to the bacteriolytic substance of 

 Cohra-\enoui. 



It results, then, from the foregoing facts that Cobnc-veiioni con- 

 tains a cytolysin, v)hich acts upon micro-organisms and is capable of 

 fixing the alexin of normal serums. 



The application of these data to the living animal is evidently 

 full of difficulties, by reason of the complexity of the substances 

 that come into play. Let us see, however, to what extent they are 

 capable of serving to explain the phenomena that are produced as 

 the result, of poisoning. 



It was observed by Kaufmann that the cadavers of animals 

 which have died from snake-bite are very rapidly invaded by the 

 bacteria of putrefaction. Welch and Ewing, referring to these 

 phenomena of rapid putrefaction in cases of death from venom, 

 explained them as being due to the loss of the bactericidal power of 

 the serum. In hot countries, even when snake-bites are not fatal, 

 they are frequently complicated by local suppuration or gangrene, 

 occasioned by micro-organisms introduced at the time of the bite. 

 The minute analysis of the phenomena of poisoning shows, in 

 reality, that the organism undergoes different modifications accord- 

 ing to the quantity of venom injected and its channel of penetration. 



When the dose of venom is rapidly lethal, whether because it 

 penetrates into the veins or because a larger amount of it is diffused 

 beneath the skin, it occasions a transient hypoleucocytosis, which 

 is, moreover, a reaction common to injections of venom, pro-peptone, 

 extracts of organs, and microbic toxins (Delezenne, Kolf). It 

 follows that blood collected a short time after the injection may 

 be totally bereft of its bactericidal power, in consequence of the dis- 

 appearance of the leucocytes, which have migrated into the organs. 



Thus it was observed by S. Flexner and H. Noguchi that 

 the serum of a rabbit, treated with 10 milligrammes of Cobra- 

 venom, showed, fifty-seven minutes after the injection, a great loss 

 of bactericidal properties. But it is impossible to conclude, from 



