THIELAVIOPSIS PARADOXA. 521 



twelve hours becomes sooty black in colour, when the central 

 vacuole can no longer be observed. They measure on the 

 average 22 x 15 [i.. 



" In many cases in this drop submerged macroconidio- 

 phores and collapsed aerial microconidiophores were seen to be 

 developed from the same hypha, thus bearing out Massee's 

 statement that micro- and macro-conidia are developed from 

 the same mycelium. The drop in question was freely exposed 

 to the diffused light of the laboratory, but not to direct 

 sunlight, consequently darkness is not necessary for the 

 formation of macroconidia. A similar development was 

 observed in several other hanging drops containing macro- 

 conidia only. 



" A hanging-drop culture was obtained containing a single 

 microconidium. The spore germinated six hours after sowing 

 and sent out a colourless septate hypha wliich soon branched. 

 The mycelium quickly extended right through the drop, and 

 its subsequent development was similar in all respects to that 

 described above in the case of the macroconidia. Aerial 

 microconidiophores were formed as before, whicli ejected 

 chains of microconidia, while the submerged hyphse formed 

 chains of macroconidia inside the drop. 



" The number of microconidia formed by one conidiophore 

 is frequently very large, as many as ninety being observed. 



" The development of the micro- and macro-conidia is 

 therefore practically identical ; had the drops not been labelled 

 it would have been impossible to 'have distinguished between 

 them." 



Howard states that he separated the macro- and micro- 

 conidia by beating up the mycelium in water and pouring off the 

 upper portion. " The macroconidia being larger and heavier, 

 subsided more quicldy than the hghter microconidia, and 

 therefore the lower layers of water were richer in the former 

 bodies." 



F. von Hdhnel. 



Sporochisma paradoxum was rediscovered, on the endo- 

 sperm of a coconut in Vienna, by Dr. F, v. Hohnel (12). He 

 considered that it was identical with Thielaviopsis ethaceticus 

 Wont, and submitted specimens to Went, who confirmed liis 



