560 FETCH : 



in the first four minutes produced a fair growtli in three days, 

 but nothing resulted from the transfers at the end of the fifth 

 and the succeeding twenty minutes. In this case, therefore, 

 tlie spores were killed by an immersion of five minutes. 



The growth of the first four cultures was somewliat slower 

 than is usual with normal spores, but not markedly so ; and 

 there was no difference between the four cultures. 



(2) Culture divided into three parts treated separately : — 



(a) Spores transferred to hanging drops by a straight 

 platinum wire after two minutes' immersion, and 

 every subsequent minute for ten minutes ; then every 

 two minutes for twenty minutes. No germination 

 in five days. 



{h) Spores transferred to flasks by a platinum loop after tAVO 

 minutes' immersion , and every minute for ten minutes; 

 then every two minutes for twenty minutes, every 

 five minutes for the next half hour, and every fifteen 

 minutes for one hour. After two days, growth in 

 Nos. 1,2, and 8; good growth in these three at the 

 end of five days. These spores therefore survived 

 immersion for two, three, and ten minutes respec- 

 tively. Total period of transfers, two hours. Growth 

 was considerably retarded. 



(c) Mycelium and spores transferred to flasks of sugar cane 

 extract . First transfer after eight minutes' immersion ; 

 subsequently every two minutes up to one hour, and 

 then at intervals of ten to fifteen minutes for two 

 hours. No growth in any in six days. Total period 

 of transfers three hours. 



(3) Culture, nine days old, instituted at the same time as 

 (2), and from the same spores, separated into three parts, 

 treated separately : — 



(a) Spores transferred to hanging drops by a straight 

 platinum ware every fifteen seconds for two minutes. 

 No germination in five days. 



(6) Spores transferred to flasks by platinum loop ; every 

 thirty seconds for six minutes. No growth in two 

 days ; microconidia in Nos. 2 and 7 after tlu'ee days. 



