THIELAVIOPSIS PARADOXA. 561 



Good growth is Nos. 2 and 7 after four days. These 

 spores therefore survived immersion for one and 

 three and a half minutes respectively, 

 (c) Mycelium and spores transferred to flasks, every thirty 

 seconds for ten minutes. Poor growth in Nos. 1,2, 

 and 3 after two days. Fair in 1, 2, 3; poor but 

 black in 4 and 5 ; still white in 6 ; nothing in 

 7 ; poor but black in 8t— after three days. Good 

 growth in 1-6 and 8, after five days. No further 

 development. These spores therefore germinated 

 regularly up to the end of three minutes' immersion ; 

 the sample taken after three and half minutes was 

 dead, but those taken after four minutes gernunated. 



At the end of six days, fresh spores of Thielaviopsis were sown 

 in several of the hanging drops and flasks in which no growth 

 liad occurred, to determine whether this was due to the amount 

 of mercuric chloride transferred with the spores. In all cases 

 these spores germinated and the fungus developed normally. 



The growth of the fungus is usually considerably retarded 

 by the previous immersion of the spores. In 3 (&) the growth 

 in four days was about equal to a normal two days' growth ; 

 while in 3 (c) the same stage was reached in three days by the 

 earlier and four days by the later transfers. • 



Summarizing the experiments with 0*1 per cent, mercuric 

 chloride, we have tlie following results : — 



(a) Spores transferred to hanging drops do not germinate 

 after an immersion of fifteen seconds. 



(6) Spores transferred to flasks germinated in no case after 

 eleven minutes' immersion. In one case no growth 

 occurred after five minutes ; in another, the majority 

 were kiUed by four minutes' immersion, but some sur- 

 vived ten minutes ; in the third case, some were killed 

 by an i mmersion of thi rty seconds , others survived one 

 minute's immersion, most were killed by one and half 

 minutes, but some survived three and half minutes. 



(c) Transfers of mycelium and spores show the same ir- 

 regularity as transfers of spores only. In one case 

 no growth was obtained after an immersion of eight 



