THIELAVIOPSIS PARADOX A. 563 



were made, extending over the next two days. Nos. 1 and 2 

 began to grow two days after the transfer, and produced a good 

 growth of macroconidia, &c., on the following day. There was 

 no growth in any of the remaining flasks, though they were kept 

 for ten days. These spores therefore survived an immersion of 

 two minutes, but were killed by an immersion of three minutes. 

 (2) Culture six days old, divided into three parts which 

 were treated separately : — 



(a) Spores transferred to hanging drops by a straight 

 platinum wire every fifteen seconds for two minutes, 

 iind every thirty seconds for the succeeding four 

 minutes. Examined two da.ys later, transfers 1-4 

 showed good growth, 5 was fair, and there was a 

 shght growth in 6 ; in the remaining ten no spores had 

 germinated. On the third day, the spores in transfers 

 7 and 8 had germinated, but there was no growth 

 in 9-16. Nothing further occurred. These spores 

 therefore survived an immersion of two minutes, but 

 were killed by an immersion of two and a half minutes. 

 Isolated spores in transfers 7 and 8 germinated. 



(b) Spores transferred to flasks of sugar cane extract by 



means of a platinum loop. Transfers made every 

 thirty seconds for five minutes, and every minute 

 for the succeeding twenty -five minutes. Growth 

 occurred in all flasks up to and including No. 13. 

 These spores tlierefore sm-vived an immersion of 

 eight minutes, but were killed by an immersion of 

 nine mJnutes (see below). 



(c) Mycelium transferred to flasks of sugar cane extract, 



every minvite for twenty minutes, then at intervals 

 of five minutes for the next forty minutes, and 

 subsequently at intervals of fifteen minutes for the 

 next six hours. All transfers up to and including 

 No. 12 grew, 13 failed, 14 and 15 grew, 16 failed, 17 

 grew ; the remainder a,ll failed. The spores of No. 17 

 thus survived an immersion of seventeen minutes. 



In 2 (a) it will be noted that the germination of the surviving 

 spores in transfers 7 and 8 was retarded by one day in com- 

 parison with transfer 6 of the same series and nearly three 



