566 PETOH : 



first, transfers were made every minute for twenty-six minutes ; 

 in the second, every minute for thirty- two minutes ; in the 

 tliird, every minute for forty minutes. As the spores were 

 killed by an immersion of fifteen minutes in the previous 

 experiment, it was expected that tliese times would be amply 

 sufficient, but contrary to this expectation all the transfers 

 grew well, and therefore no result was arrived at. 



(3) Culture, six days old, divided into three parts which 

 were treated separately : — 



(a) Spores transferred to hanging drops by means of a 

 straight platinum wire. Transfers made every five 

 minutes for the first hour, every ten minutes for the 

 second hour, and every fifteen minutes for the fifth, 

 sixth, and seventh hours. No spores germinated 

 after the eighth transfer. They, therefore, survived 

 an immersion of forty minutes, but were killed by 

 an immersion of forty-five minutes. Germination of 

 isolated spores occurred in all transfers up to the 

 eighth ; in the latter, 50 per cent, of the isolated 

 spores and 53 per cent, of the spores in groups 

 germmated within twenty-four hours. 



(6) Spores transferred by platinum loop to flasks of cane 

 extract. Transfers as above. All transfers grew ap 

 to No. 20, i.e., after an immersion ol two hours 

 twenty minutes ; all subsequent transfers failed. 

 Unfortunately, No. 21 was transferred only after an 

 immersion of four hours and forty-four minutes. 



(c) MyceHum and spores transferred by platinum wire to 

 flasks. First transfer after an immersion of one 

 hour, then every ten minutes for the second hour, 

 every fifteen minutes for the fifth, sixth, and seventh 

 hours, then everj'' hour to the end of the twelfth hour, 

 and again every liour from the twentieth to the 

 twenty-fifth hour. As in the case of (6) all the 

 transfers grew up to No. 20. The death point hes 

 therefore between two hours twenty Iminutes and 

 four hours and forty-four minutes. 



(4) The last experiment was repeated with another 

 culture, six days old, in the expectation of obtaining a closer 



