THIELAVIOPSIS PARADOXA. 569 



with that of carbohc acid, though the former is the more 

 efficacious in inliibiting germination. 



(2) A flask culture, six days old, was divided into three 

 parts, which were shredded in 5, 4, and 3 per cent, copper 

 sulphate, respectively. Transfers of fragments of mycelium 

 containing spores were made every twenty-four hours to 

 flasks of sugar cane extract. The experiment was prolonged 

 for one hundred and thirteen days. From the 5 per cent, 

 solution all the transfers grew until the forty- fifth day ; 

 subsequently growth occurred in the tra.nsfers on the forty- 

 ninth, fiftieth, fifty-first, fifty-fourth to fifty-seventh, sixty- 

 second, sixty-sixth, sixty-seventh, seventy- third, seventy-fifth, 

 and eighty-second days. In the eighty-second transfer, the 

 spores germinated in five days and produced a fair growth in 

 eight days, as compared witli two days from untreated spores. 

 From the 4 per cent, solution, all the transfers grew until the 

 sixty-fifth day ; failures occurred on the sixty-fifth, seventy- 

 sixth, eighty-first, eighty -second, eighty-fourth, eighty -sixth, 

 eighty -seventli. ninety-second, ninety-third, ninety-fifth to 

 ninety-eighth, and one liundredth days ; the one-hundred and 

 first transfer was the last that grew. From the 3 per cent, 

 solution, the first failure occurred on the forty-fifth day, as in 

 the case of the 5 per cent, solution ; further failures occurred 

 on the forty-ninth, fifty-second, fifty-seventh, sixby-firsfc, 

 sixty-third, sixty-sixth, sixty-eighth, seventy-first to seventy- 

 fourth, seventy-sixth to eighty-fourth days ; the eighty-fiftli 

 was the last transfer which grew. 



(3) A culture, eight days old, was divided into five parts, 

 which were shredded in 1, 2, 3, 4, and 5 per cent, copper 

 sulphate respectively. The mixtures were shaken up and 

 allowed to stand until tlie mycelium had sunk to the bottom. 

 The liquids with the suspended spores were then decanted into 

 other bottles, and fresh copper sulphate solutions of the same 

 strength were added to the mycehum. The bottles containing 

 the latter were put aside, and spores were transferred every 

 twenty-four hours by means of a platinum loop from the 

 bottles which contained spores only. The experiment was 

 continued for twenty-eight days. From the 5 per cent, 

 solution, the first and second transfers failed, but the third 



