570 FETCH : 



grew ; this was the oiily transfer wliich grew. From the 4 per 

 cent, solution, the first, second, fifth, and sixth gi'ew. From 

 the 3 per cent, solution aU grew, up to and including the 

 tenth ; the eleventh to the sixteenth failed, but the seventeenth 

 grew. From the 2 per cent, solution aU grew up to and 

 including the eleventh ; the twelfth and thirteenth failed, but the 

 fourteenth grew. From the 1 per cent, solution all grew up to 

 and including the ninth ; the tenth failed, but the eleventh grew; 

 the twelftli and tliirteenth failed, but the fourteenth grew ; 

 the fifteenth to the nineteenth failed, but the twentieth grew. 



The samples of mycelium and spores, which had been put 

 aside in copper sulphate solutions, were tested by transference 

 on the thirtieth and following days. No growth was obtained 

 from the 4 and 5 per cent, solutions. Growth from the other 

 three solutions occurred up to and including the seventieth 

 day, after which the experiment was discontinued. 



(4) This experiment was repeated with a culture six days 

 old, and 4 and 5 per cent, solutions of copper sulphate. As 

 some of the spores were dead in the 5 per cent, solution after 

 twenty-four liours in experiment 3, transfers were made at 

 intervals during the first day. The first failure of the spores 

 transferred from the 4 per cent, solution occurred on the 

 fourth day ; the transfers grew on the fifth to the eighth day, 

 but failed on the ninth ; they gi-ew on tlie tenth but failed on 

 the eleventh and twelfth ; the transfer on the thirteenth day 

 was the last that grew. From the 5 per cent, solution all the 

 transfers grew, up to and including that on the fifth day. 

 The experiment was continued for twenty days. 



As in experiment 3, the myceHum and spores which had 

 been kept in separate bottles of copper sulphate solution 

 were also transferred. From the 5 per cent, solution, ""growth 

 occurred in transfers on the twenty-fourth, twenty-ninth, 

 fortieth, and fiftieth days, no intermediate transfers being 

 made ; no further test was made until the sixty-sixth day, 

 when, and on subsequent days, all the spores were found to bo 

 dead. From the 4 per cent, solution growth occurred on the 

 sixty-sixth day, and in various transfers up to and including 

 the ninetieth day ; a transfer on the one-hundiedth day failed. 

 The experiment was not continued furtlier. 



