61 



Later on the difference was less distinct, partly because the 

 alcaline rice turned acid. The faculty to produce the red colour 

 was strongest in strain VIII and XII, which formed even red 

 mycelium when cultivated on wood; strain IV on the other 

 hand showed the red colour on alcaline rice only. 



The 9 strains of Fusarium theohromae were indifferent to the 

 reaction of the medium; a red colour was never produced; the 

 mycelium was mostly olive-coloured, more yellowish when 

 young and darkening, almost quite black when old. 



Perithecia. Not all of the 15 strains formed perithecia. Fu- 

 sarium colorans (strains IV, VIII, IX, X, XI, XII) never did; 

 from the 9 strains of Fiisarium theob7vmae only the strains I, 

 II, III, IV and XllI, but these 5 always did, when cultivated 

 on wood (twigs of cacao). On rice or agar perithecia never 

 appeared. 



The 9 strains of Fusarium theohromae were absolutely iden- 

 tical in all respects, only the 5 above-mentioned strains had 

 the faculty to produce perithecia in pure culture, the 4 other 

 ones not. Perhaps this observation affords an indication, that 

 it is always advisable, to take more strains in cultivation, 

 when trying to obtain higher fructications of fungi in pure 

 culture ^). 



With the object of proving conclusively that the different 

 fructifications belonged to the same species, pure cultures were 

 made of strain II, starting with one ascospore. To this end one 

 ascospore was isolated with the aid of the "Tuschepunktver- 

 fahren" and cultivated under microscopical control. These cul- 

 tures produced the^ same Fiisarium-iii^OYes aud, after some weeks, 

 the same Nectria-pevithecm. 



The perithecia with asci and ascospores, obtained in this 

 way, are reproduced on plate XL 



1) Individual differences of this kind are by no means uncommon. I mention only 

 Corlicium javanicum Zimm. Rant, when inoculating Cinchona with 2 different 

 strains of Corticium, obtained the iVecator-fructification 33 times with the first one, 

 and only once with the other one (Bull. IV. Dep. de I'Agric., de Find, et du Comm 

 aux Indes Neerl. 1912j. 



