75 



cells (in) which have been found to occur generally in the 

 other Marattiaceae as well as in the Ophioglossacae. In the 

 empty antheridium these m-antle-cells project strongly into 

 its cavity, and they are no doubt important factors in the 

 dehiscence of the ripe antheridium. 



The spermatozoids are larger than those of either Angiopterls 

 or Marattia, both of which were examined for comparison. 

 The material which was used, while excellently fixed with one 

 per cent chromicacid, did not stain very satisfactorily with the 

 double stain of saffranin and gentian-violet, but nevertheless 

 the main points of development could be followed and agreed 

 very closely with corresponding stages of the spermatogenesis 

 of OpJiioylossum which I have recently described. (Studies on 

 the Ophioglossaceae, Ann. du Jardin Botanique de Buitenzorg, 

 1907). . 



Although not staining very strongly, the two blepharoplasts 

 (PI. VIll, Fig. 19/>) could be seen in the cell previous to its final 

 division into the two spermatocytes. After this final division the 

 nucleus of the spermatocyte appears coarsely granular and, as 

 usual, no nucleolus is apparent. In favorable preparations the ble- 

 pharoplast, a round body staining rather deeply, may be seen 

 lying near the nucleus. Later the blepharoplast assumes a curved 

 form and becomes mucli extended, and from it the cilia begin 

 to develop before the nucleus has materially changed its shape. 

 The latter now becomes slightly pointed at one end and begins 

 to extend itself so that it appears somewhat crescent-shaped, 

 very much as is the case in (Jp]iW(jlos.-mni, and as has been 

 described by Belajeff and others in Equlsetuin and many ferns. 

 The blepharoplast in the meantime becomes still more elongated 

 and strongly coiled, and the cilia increase in length. The nucleus 

 in Kaul/K.ssia becomes less elongated than is usual in the ferns 

 and also less than is found to be the case in Anglopterls. In 

 this respect as well as in its larger size it more nearly resem- 

 bles Ophioglos.sum than Anglopteris. The granular appearance 

 of the nucleus is maintained until the spermatozoid is almost 



