158 



tangos is quite variable. Sometimes (Fig. 58) the filaments 

 are nearly straight, runriiDg from cell to cell and branching 

 sparingly. Sometimes (Fig. 56) a cell is completely filled with 

 a dense tangle of liyphse, while in other cases there are 

 sack-like swellings of very irregular form (Fig. 57). Not 

 infrequently quite regular, nearly globular, bodies are seen, 

 recalling the young oogonia of the Peronosporeae (Fig. 59). 

 They at first contain comparatively few nuclei scattered 

 through the reticulately arranged cytoplasm. In the older ones 

 the nuclei are very numerous and decidedly larger (Fig. 61). 

 It looked in some cases as if this was a preparation for the 

 formation of spores, but no certain evidence of the discharge 

 of such spores could be seen, although in several instances 

 there was an appearance noted which might point to this — 

 masses of nuclei apparently free in the cell of the host. Struc- 

 tures resembling the ''conidia" described by Jeffrey for Botry- 

 chium (loc. cit. p. 12) were seen and were probably the same 

 thing. In most of the cells infested by the fungus was noted 

 the peculiar aggregated appearance so frequently described 

 (Fig. 56). Here it is clear that the irregular clumps are formed 

 by the aggregation and breaking clown of the starch granules 

 found in the young cells. The nucleus of the host cell appears 

 to be little affected by the growth of the fungus, and can 

 usually be found quite unchanged even in those cells which 

 are almost completely filled l)y the endophyte. 



The endophyte, as in the other forms that have been studied, 

 is quite absent from the apical region of the branches. In the 

 old tissues of the gametophyte it is often present in great 

 abundance. There is a more or less well marked zone inside 

 the superficial tissue where the fungus is especially abundant 

 (Figs. 45 — 16) , while the central region is quite free from 

 the fungus or only slightly infested. However, the limits 

 of the infested zone are not very clearly marked, and any 

 cell of the older tissue of the gametoiDhyte may harbor the 

 fungus. 



