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period of the appearance of the latter. Tn the globular type of 

 embryo the rudiment of the second root is very early apparent 

 (Fig. 1 25) and it looks as if it were formed quite independently 

 of the primary root. In some cases (Fig. 41) the primary root 

 may attain a length of several centimeters, and may even 

 begin to form secondary roots before the second root emerges. 

 Tn other cases (Fig. 42) the two roots grow in opposite 

 directions and seem to be equally massive. 



In searching for young germ plants, no very small ones were 

 seen ~ the smallest leaves being some 20 centimeters or more 

 in length, and these were invariably connected with a root bud 

 and in no cases with the prothallium directly. Not realizing 

 that these were the young sporophytes growing as buds upon 

 the root of the embryo, no effort was made at the time to 

 trace their connection with the latter, and unfortunately it 

 therefore cannot be stated just when they make their ap- 

 pearance. The primary root, although attaining a length of 

 10 centimeters or more, in no cases showed any signs of the 

 leaf-bearing bud in any specimens that were collected. Rootlets 

 were observed in some instances (Fig. 41) and it is not impos- 

 sible that there may be an extensive development of a root- 

 system before the first leafy bud is formed. This is quite in 

 harmony with the large development of the roots in 0. vulgatum, 

 where Bruchmann believes that it may be eight or ten years 

 before the first foliage leaf appears above the ground. In 

 0. vulgatum, however, this leaf arises from the original stem 

 apex derived directly from the embryo and not from an adven- 

 titious bud. 



The terrestrial species of Ophioglossum collected at Buiten- 

 zorg (and this was also the case with the one collected at 

 Hakgala) differ very much from 0. pendulum in the character 

 of the young sporophyte, but as already has been stated, the 

 early stages of the embryo could not be followed. The type of 

 the sporophyte is closely like that of 0. pedunculosum described 

 by Mettenius, and so close is the resemblance that it would 

 seem to confirm the close relationship of this species and pos- 



