186 



where there is no large amount of oil developed, it is not 

 likely that this is a prominent function of the endophyte. 



The infection of the young sporophyte takes place in both 

 0. moluccanum and (). pendulum at an early period, and at the 

 time the young sporophyte In-eaks through the prothallial 

 tissue the endophyte is well established. It first appears in the 

 foot, and from there follows the development of the root. 

 There is no differeuce in appearance between the endophyte 

 in the gametophyte and that in the root of the sporophyte; 

 although the actual passage of the fungus from the prothallial 

 tissue into the young sporophyte was not detected, there seemes 

 little question that the infection of the sporophyte is mainly 

 from the prothallial tissue, and not from the mycorhiza outside, 

 as Bruchmann thinks is the case in 0. vuhjatum. 



The presence of an endophytic fungus is not confined to a 

 chlorophylless gametophyte. They have also been observed in 

 a large number of liverworts (Humphrey — The Development 

 of Fossomhronia longmta, Ann. of Bot. XX : 87, 1906). In looking 

 over some old preparations of Osmunda the writer found a 

 prothallium of 0. cinnamomea in which there was an abundant 

 endophyte closely resembling that found in the Ophiogiossaceae. 

 Whether this is a common occurrence in Osmunda remains to 

 be seen. In the green gametophyte it may be assumed that 

 the symbiotic nature of the fungus is more or less questionable, 

 but it is important to find that such an endophyte may exist 

 iu a green prothallium, as this would help to explain its 

 presence in the saprophytic gametophyte of Ophioglossum. It 

 will be interesting to discover whether or not such endophytic 

 fungi also occur in any Marattiaceae or Gleicheniaceae. 



THE SPECIES OF OPHIOGLOSSUM IN JAVA. 



Racibohski (Die Pteridophyten der Flora von Buitenzorg, 1898) 

 records only tw^o species of Ophioglossum in Java — 0. moluc- 

 canum ScHLECT. and 0. i)enduluin L. Later (Die Fame von 

 Tegal, Natuurkundig Tijdschrift, LIX) he calls attention to some 



