188 



species, perhaps nearer to the peculiar 0. simplex Ridley than 

 to 0. pendulum. 



At Tjibodas, a terrestrial species of Ophioglossivm was very 

 abundant, but all attempts to grow the spores were unsuccessful, 

 nor could any prothallia be procured, although repeated search 

 was made for these. This species belongs to the reticulatiun 

 group, and closely resembles the species collected in Ceylon, 

 and may be the same. Raciborski does not seem to have 

 collected this, as he makes no reference to any species from 

 Tjibodas except 0. pendulum. 



It is evident that the terrestrial species of Ophioglossum of the 

 Indo-Malayan region are very much in need of a careful revision. 



SUMMARY. 



The mos^. important points brought out in the foregoing 

 paper may be briefly summarized as follows: 



1. The spores of 0. m.oluccanum germinated freely and 

 promptly, but did not proceed beyond a four-celled stage, 

 owing, apparently, to failure to become associated with the 

 mycorhizal fungus. Germination in 0. pendtdum was slower, 

 but in a number of cases the association with the fungus was 

 established, and growth continued. Prothallia of twelve to 

 thirteen cells were obtained in this species. 



2. No trace of chlorophyll was found in O. pendidum, but 

 in 0. moluccanum some of the young prothallia developed a 

 few chloroplasts. 



B. Adult prothallia were found in 0. moluccanum and 0. pen- 

 dulum. Also in an undetermined species from Hakgala, Ceylon. 



4. The gametophyte of all the species is subterranean and nor- 

 mally destitute of chlorophyll, and radial in structure as described 

 by Mettenius, Bruchmann and Lang. It is very large in 0. pen- 

 dulum, and apparently capable of unlimited reproduction by 

 means of detached buds. In O. moluccanum it is short lived, 

 probably living only for a single season. 



5. The antheridium of all the forms examined agrees in its 

 development with the description given by Lang and Bruchmann. 



