420 Agricultural Experiment Station, Ithaca, N. Y. 



the interior was partially or wholly tilled with large grannies of an 

 orange color tinged with green. In some cases constrictions were 

 to be seen in the larger bodies, and always at the point where the 

 septum was located (Fig. 1). 



A dilution culture was made on May l^th, and in two days one 

 or more slender germ-tubes had been put out at one or both ends 

 of the hyphal body. After five days the growth stopped. Pieces 

 of agar, containing live and growing bodies, were removed and 

 placed in acidified agar, but no further growth took plac'\ On 

 May 16th, a red cedar, Janiperus virgimana, was found infested 

 with another scale, Lecanimn Jietcheri,, and as some of these ap 

 peared very much like the ones on maple, they were also examined. 

 Bodies closely resembling those in the first scale were found. They 

 differed only in being larger and once or twice septate (Fig. 4).* 

 A dilution culture was made and germination took place as in the 

 first instance, with, however, a more vigorous growth. Fig. 5 shows 

 the bodies after one day; Fig. 7 a portion of the mycelium after 

 two days. Septa and a few small vacuoles appear about this time. 

 Branching occurs quite profusely and irregularly. Fig. 6 shows a 

 portion of the mycellium after five days. Many large and regularly 

 placed vacuoles are present and the threads are constricted between 

 the vecuoles. Small pieces of agar containing this growth were 

 transferred to tubes of acidified agar, and some were also transferred 

 to tubes containing potato steeped in a strong infusion of Lecani- 

 ums. Out of a large number of tubes, two showed a growth of 

 very fine, white, cottony filaments which in time became quite 

 dense and somewhat matted. On exatnination this growth is found 

 to consist of long, fine filaments bearing, at irregular intervals, 

 flask-shaped sterigmata placed at right angles to the parent thread, 

 and which taper to a fine point and bear usually one oval or ovate 

 conidium from 3 to 4 microns in size. The thread is filled with 

 hyaline protoplasm containing small granules (Fig. 9).- Sometimes 

 there is a short side branch from the tip of the sterigma which 

 bears a second spore (Fig. 9). From these cultures conidia were 

 transferred to tubes containing sterilized sticks of elm covered with 

 coccids. A vigorous growth was in this way obtained. About this 



*Siuce that time, specimens identical with these have heen found on maple 

 and specimens similar to the ones first found were also seen in L. fletcheri. 

 It seems almost certain that the two forms are specifically one, as intergrading 

 forms are present in both cases. 



