HOSTON. — CULTURE STUDIES OF FUNGI. 285 



the hyphae are closely packed with oil globules which escape into the 

 water when the filament is ruptured, anil might be mistaken for spores. 



The bulbils. — A short lateral branch coils up, usually making one 

 or two turns, rarely more, and frequently less than two, and divides 

 into a number of short cells from which secondary branches are pro- 

 duced, or from which individual cells are formed by budding (Figures 

 7-8, Plate 8). In either case, spherical cells which gradually increase 

 in size, are developed, and the lateral walls adhere closely to each 

 other. The original coil, the cells of which in the meantime have 

 become much enlarged and rilled with granular material and oil 

 globules, is thus eventually completely surrounded. At maturity 

 three or four large central cells may be distinguished which have 

 become angular by pressure, surrounded by a layer of fairly regular 

 cells which are also usually somewhat angular except the outer walls. 

 It often happens that when one turn is made by the primordial coil, 

 the secondary branches begin to form, while at other times two or 

 more turns are formed before this happens. Between these two 

 extremes a number of variations are found. Not infrequently the 

 lateral branch becomes divided into four to eight cells and may or 

 may not be coiled at the end, and from these, secondary branches 

 are produced which coil around each other and around the original 

 branch, dividing and subdividing, the lateral edges eventually adher- 

 ing closely, and producing a more or less elongated bulbil (Figures 

 4-6, Plate 8). This process also inhibits the further growth of the 

 coil. An extreme instance of this is shown in Figure 6, Plate 8, 

 where several cells are seen to take part in the formation of lateral 

 branches. Bulbils formed from a primordium of this type are elon- 

 gated, irregular, and larger than those formed in the usual way. 



Although this species was grown on a great variety of nutrient 

 media, it could not be induced to develop any perfect form or even 

 another imperfect type. 



Papulospora parasitica nov. comb. 



Syn.: Helicosporangium parasiticum Karsten. (nee Eidam.) 



Plate 5, Figures 1-17. 



Mycelium septate, white, flocculent; bulbils light brown, nearly 

 spherical, 15-21 n in diameter, with a single large central cell sur- 

 rounded by a single layer of empty colorless cells; primordium a 

 spiral, coiled crosier-fashion. 



